摘要
针对中国大豆灰斑病 1号生理小种 ,以抗所有生理小种的品系东农 4 0 5 6 6为母本 ,以感 1号生理小种的品种东农 4 10为父本配制杂交组合 ,杂交得到F2 代后连续自交 3代得到F5代群体。该群体经人工接种灰斑病 1号生理小种后 ,利用BSA法对 5 0 0个SSR标记进行筛选 ,其中 3个标记Satt5 6 5、SOYGPATR和Satt396在抗、感池间表现出稳定的多态性 ,并且在F2 代个体中表现出抗性与多态性协同分离的趋势。这 3个标记与抗性基因的连锁顺序为Satt5 6 5 -SOYGPATR-Hrcs1-Satt396 ,它们与抗性基因的连锁距离分别为 12 .7cM、6 .5cM、14 .7cM。推测抗大豆灰斑病 1号生理小种的基因可能位于C1连锁群上。
In aim to find the SSR markers linked to resistance gene against frogeye leaf spot in Chinese soybean, bulked segregant analysis was applied to F 5 populations derived from cross between the resistant line Dongnong40566 and the susceptible line Dongnong410, self-crossing for three generations after F 2.Three SSR markers including Satt565,SOYGPATR and Satt396 were identified from 500 SSR markers, the linkage order is Satt565-SOYGPATR-Hrcs1-Satt396, and the distance to the resistance locus is 12.7, 6.5 ,14.7cM respectively. These three markers and the resistance locus were maped to soybean linkage group C1.
出处
《大豆科学》
CAS
CSCD
北大核心
2004年第3期169-173,共5页
Soybean Science
基金
国家"8 63"项目资助
关键词
大豆
灰斑病
抗性基因
SSR标记
品系
杂交组合
Soybean (G.max)
Frogeye Leaf Spot(Cercospora sojina Hara)
Resistant gene
SSR marker
