摘要
采用ISSR分子标记对金花茶的4个自然分布居群的126份样品的遗传多样性水平进行了研究。用12条引物,共检测到105个清晰的扩增位点,其中多态性位点79个,多态位点百分率(PPB)为75.24%。采用POPGENE软件进行分析,结果表明:居群总的Nei's基因多样性指数为0.2302,Shannon信息多态性指数为0.3502,金花茶总的遗传多样性水平较高。但金花茶居群内的遗传多样性相对较低。基因分化系数为0.5752,遗传变异主要存在于居群间。用NTSYS软件对样品进行UPGMA聚类分析,结果4个居群的样品各自聚在一起,而金花茶两间断分布区区内的居群又各自聚在一起。金花茶4个居群间的遗传距离与地理距离呈显著的正相关(r=0.68261,P=1.0000)。
Camellia nitidissima Chi (Theaceae)is an important horticultural and economic species,distributed in a narrow region of southern China and northern Vietnam.One hundred and twenty-six individuals from four populations of C.nitidissima were analyzed by ISSR (inter-simple sequence repeat) markers to detect the genetic variation among and within populations.Twelve primers produced a total of 105 scorable markers,of which 79 (75.24%) were polymorphic.As analyzed by the software POPGENE and NTSYS,the genetic diversity of C.nitidissima was high,with Nei's gene diversity of 0.2302 and Shannon's Information Index of 0.3502.In contrast,the genetic diversity with each population was low.The coefficient gene differentiation (G_(st)) was 0.5752,indicating more differentiation among populations than within populations.UPGMA cluster based on Nei and Li genetic similarity formed 4 clades correspongding to the four populations.The Mantel test revealed that a positive correlation (r=0.68261,P=1.0000) existed between genetic distance and geographical distance.
出处
《武汉植物学研究》
CSCD
北大核心
2005年第1期20-26,共7页
Journal of Wuhan Botanical Research
基金
国家自然科学基金资助项目(39860011)。
关键词
金花茶
ISSR
遗传多样性
Camellia nitidissima Chi
ISSR
Genetic diversity
