摘要
利用ISSR(inter simplesequencerepeats)技术比较了 2 4份中国半冬性、瑞典冬性和瑞典春性油菜的遗传多样性。 2 0个引物扩增出了 12 5条多态性带。UPGMA聚类分析将 2 4份材料分为 3组。第一组为 6份瑞典冬性和 8份中国半冬性材料 ,第二组为 2个中国半冬性材料湘油 15号和保 81,第三组为 8份瑞典春性材料。主成分分析结果与聚类分析结果相似 ,表明研究所用中国半冬性、瑞典冬性和瑞典春性 3类材料彼此间区分明显 ,中国半冬性油菜与瑞典冬性油菜的遗传关系比与瑞典春性油菜的关系近。结果显示 。
Genetic diversity of 24 Chinese weak-winter, Swedish winter and spring B.napus accessions by inter-simple sequence repeats (ISSRs) was compared. By cluster analysis (UPGMA) based on 125 polymorphsm bands amplified with 20 primers, all 24 accessions were divided into three groups. Six Swedish winter lines and eight Chinese weak-winter lines were in the group I and two Chinese weak-winter lines ‘Xiangyou15’ and ‘Bao811’ were in the group II. The third group were eight Swedish spring lines. Principal co-ordinates analysis (PCO) showed similar groupings to cluster analysis. Results from cluster analysis and PCO analysis showed very clearly that Chinese weak-winter, Swedish spring and winter accessions were distinguished from each other and Chinese weak-winter accessions in this study were genetically closer to Swedish winter accessions than to Swedish spring accessions, and that Chinese weak-winter accessions had larger diversity than Swedish spring or winter accessions. As this study indicated above, ISSR is a suitable and effective tool to evaluate genetic diversity among rapeseed germplasms.
出处
《中国农业科学》
CAS
CSCD
北大核心
2003年第11期1403-1408,共6页
Scientia Agricultura Sinica
基金
欧盟资助项目 (IC18 CT97 0 172 )
