摘要
目的用RAPD和ISSR方法分析了4种正品龙胆的亲缘关系及遗传多样性,为正品龙胆的品种鉴定及栽培育种提供了分子生物学依据。方法优化RAPD及ISSR的PCR反应体系,对产物琼脂糖凝胶电泳结果进行数据统计。结果从100个RAPD引物和32个ISSR引物中分别筛选出10个RAPD引物和4个ISSR引物。通过遗传距离系数UPGMA聚类法分析数据,构建类聚关系树系图。结论RAPD及ISSR的PCR反应体系可以获得理想的实验结果,适用于龙胆品种遗传多样性及亲缘关系的分析。
Objective To analyze the sibling relationship and genetic diversity of four certified Radix Gentianae (RG) species by RAPD and ISSR methods. It provided molecular biological proof for species identification and breeding of certified RG. Methods PCR reaction systems of RAPD and ISSR were optimized, and the agarose gel electrophoresis results were analyzed into statistic data. Results Ten RAPD primers and four ISSR primers were selected respectively from 100 RAPD primers and 32 ISSR primers. The statistic data were analyzed to construct cluster analysis dendrogram by genetic distance UPGMA method. Conclusion The PCR reaction systems of this experiment gets ideal RAPD and ISSR results that suit to the analysis of certified RG species in genetic diversity and sibling relationship.
出处
《中草药》
CAS
CSCD
北大核心
2005年第1期100-103,共4页
Chinese Traditional and Herbal Drugs
基金
黑龙江省教委重大资助项目(9551Z002)
