摘要
以连云港花果山野生蕨菜干粉为原料,通过正交试验选择最佳提取条件,用分光光度法测定总黄酮和多糖的含量。结果表明:蕨菜中多糖提取的最优条件为蕨菜与水料液比1:20(g/mL)、提取温度90℃、提取时间1.5h、提取次数2次。新鲜蕨菜干粉中多糖含量为1.39%,三月枯蕨菜干粉中多糖含量为7.08%;提取蕨菜中黄酮类物质的最优条件为蕨菜与70%乙醇比1:10(g/mL)、提取温度90℃、提取时间2h、提取次数2次,测得新鲜蕨菜干粉中总黄酮含量为4.90%,三月枯蕨菜干粉中总黄酮含量为3.11%。
The optimal extraction processing of polysaccharides and total flavonoids from wild Pteridium aquilinum powder collected from Huaguo Mountain of Lianyungang were explored by orthogonal experiments. The contents of total flavonoids and polysaccharides were determined by spectrophotometry. The optimal extraction conditions for polysaccharides were material-liquid ratio of 1：20, extraction temperature of 90 ℃, extraction repeat number of 2 and extraction time of 1.5 h each time. Under the optimal extraction conditions, the contents of polysaccharides in fresh Pteridium aquilinum and dry Pteridium aquilinum were 1.39% and 7.08%, respectively. The optimal extraction conditions for total flavonoids were material-70% ethanol ratio of 1：10, extraction temperature of 90 ℃, extraction repeat number of 2 and extraction time of 2 h each time. Under the optimal extraction conditions, the contents of total flavonoids in fresh Pteridium aquilinum and dry Pteridium aquilinum were 4.90% and 3.11%, respectively.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2010年第24期124-127,共4页
Food Science
关键词
多糖
黄酮
蕨菜
polysaccharide
flavonoids
Pteridium aquilinum
