摘要
利用分光光度法,研究蕨老茎叶提取液体外清除亚硝酸盐的能力。在单因素实验基础上,利用Design-Expert Software8.06设计了响应曲面实验,考察了乙醇体积分数、提取时间、液固比以及提取温度四因素对蕨老茎叶提取液清除亚硝酸盐效果的影响。蕨老茎叶中清除亚硝酸盐有效成分的最佳提取工艺条件为:液固比(mL/g)为69∶1,用体积分数71%的乙醇于81℃水浴回流90 min,此时提取液对亚硝酸盐的最大清除率为70.21%。模型预测对亚硝酸钠的清除率为69.50%。
The nitrite scavenging capacity of extracts from Pteridium aquilinum var. latiusculum was evaluated in vitro by spectrophotometric method. On the basis of single factor tests, response surface analysis was designed by Design-Expert Software 8.06. The effects of extraction time, ethanol concentration, ratio of liquid to solid, and temperature on the nitrite elimination were investigated. The results show that the optimal extraction conditions were as follows：with 71% ethanol as the extraction solvent and a ratio of liquid to solid of 69 mL/g,the extraction was conducted at 81 ℃ for 90 min. The predicted rate of elimination of sodium nitrite was 69.50%, close to the practical elimination of sodium nitrite, which was 70. 21%.
出处
《精细化工》
EI
CAS
CSCD
北大核心
2013年第6期652-655,共4页
Fine Chemicals
基金
甘肃省教育厅研究生导师资助项目(1009-09)
甘肃省高校河西走廊特色资源利用省级重点实验室科研资助项目(XZ1203)~~
关键词
蕨浸提液
清除率
亚硝酸钠
响应面法
食品与饲料用化学品
Pteridium aquilinum var. latiusculum extracts
elimination rate
sodium nitrite
responsesurface methodology
food and feedstuff chemicals
