摘要
目的通过观察杜仲总黄酮对人肺腺癌细胞H1299细胞增殖的影响,筛选杜仲抗肿瘤的药效成分。方法乙醇分段法提取杜仲皮中总黄酮,以25μg/ml、50μg/ml、100μg/ml、200μg/ml浓度分别加入H1299细胞中,用MTT法检测H1299细胞的增殖情况。结果杜仲总黄酮为25μg、50μg、100μg、200μg/ml剂量组的OD490吸光值均降低,与对照组相比,有统计学意义(P<0.05)。结论杜仲总黄酮能直接抑制体外培养的人肺腺癌细胞H1299细胞增殖。
Objective To investigate the effect of total flavone in Eucommia bark on proliferation of the human lung adenocarcinoma cell H1299.Methods The total flavone were extracted from Eucommia bark by ethanol of differential concentrations（from 60%~90%）,and the H1299 cells was cultured with total flavone of differential concentrations at 25 μg/ml,50μg/ml,100 μg/ml and 200 μg/ml in vitro.The proliferation effects of the H1299 cells were detected by MTT.Results The cells cultured with the total flavone from 25 to 200 μg/ml had lower optical absorption at 490 nm.They were obviously different from control groups（P0.05）.Conclusions The total flavone of Eucommia bark could inhibit the proliferation of H1299 cells in vitro.
出处
《遵义医学院学报》
2010年第3期218-219,共2页
Journal of Zunyi Medical University
关键词
杜仲总黄酮
H1299细胞
肺腺癌
细胞增殖
total flavone of Eucommia bark
H1299 cell
lung adenocarcinoma
cell proliferation
