摘要
目的:研究参芪扶正注射液的药效物质基础。方法:利用多种色谱方法进行成分分离,根据物理化学性质和波谱学数据分析对化合物进行结构鉴定。结果:从注射液原料粉中分离得到12个化合物,分别鉴定为芒柄花素(formononetin,1)、芒柄花素-7-O-β-D-葡萄糖苷(formononetin-7-O-β-D-glucopyranoside,2)、毛蕊异黄酮(caly-cosin,3)、毛蕊异黄酮-7-O-β-D-葡萄糖苷(calycosin-7-O-β-D-glucopyranoside,4)、9,10-二甲氧基紫檀烷-3-O-β-D-葡萄糖苷(9,10-dimethoxypeterocarpan-3-O-β-D-glucopyranoside,5)、黄芪甲苷(astragaloside Ⅳ,6)、异黄芪皂苷Ⅰ(isoastragaloside Ⅰ,7)、异黄芪皂苷Ⅱ(isoastragaloside Ⅱ,8)、乙酰黄芪皂苷Ⅰ(acetylastragaloside Ⅰ,9)、5-羟甲基糠醛(5-h.roxymethyl-furaldeh.e,10)、丁香苷(syringin,11)、果糖(fructose,12)。结论:该制剂以黄酮苷及皂苷为主要药效成分。
Objective： To isolate and identify efficienct components of Shenqi Fuzheng Injection. Methods： Compounds were isolated by AB-8 macroporous resin, silica gel column chromatography, gel column and recrystallization. Physicochemical characters and spectral analysis were employed to identify structures of compound isolated. Resuits： Twelve compounds were isolated and identified as formononetin （1）, formononetin-7-O-β-D-glucopyranoside （2）, calycosin （ 3 ） , calycosin-7-O-β-D-glucopyranoside （ 4 ）, 9, 10-dimethoxypeterocarpan-7-O-β-D-glucopyranoside （5）, astragaloside Ⅳ （6）, isoastragaloside I （7）, isoastragaloside Ⅱ （ 8 ）, acetylastragaloside Ⅰ （9）, 5-hydroxymethyl-furaldehyde（10）, syringin（ 11 ）, fructose （12） . Conclusion： Flavonoid glycoside and saponin are mostly efficienct components of the injection.
出处
《中国现代中药》
CAS
2009年第8期19-22,43,共5页
Modern Chinese Medicine
基金
辽宁省教育厅科学研究项目(20060879)
关键词
参芪扶正注射液
药效成分
结构鉴定
Shenqi Fuzheng Injection
Efficienct components
Structural identification
