摘要
通过实验筛选得到适合三聚氰胺的荷移剂——茜素红,并对茜素红荷移光度法测定三聚氰胺的条件进行了探讨,获得的最佳条件为:三聚氰胺水溶液与茜素红乙醇溶液中性下反应,温度20℃,pH 6.5～8,时间30 min,得n_(三聚氰胺):n_(茜素红)=1:1紫红色荷移络合物,在λ_(max)=560 nm下,测得摩尔吸光系数ε=7.44×10~4L·mol^(-1)·cm^(-1),在6.1×10^(-5)～1.22×10^(-3)mol·L^(-1)内符合比尔定律,线性方程为A=1.406×10^(-5) c-0.0463(r=0.9967)。该法对市售生姜、黄豆和奶粉中三聚氰胺含量测定的结果与GB/T 22388—2008 HPLC法一致,回收率71.1%～85.5%,RSD为2.9%～4.0%。此法操作易掌握,灵敏度高,结果较为满意,但样品的预处理稍繁琐。
The charge transfer reaction of melamine in some foods with alizarine red in neutral solution formed a 1：1 complex with apparent molar absorptivity ofε= 7.44×10 L·mol^（-1）·cm^（-1）（atλ_（max）= 560nm）.Beer＇s law was obeyed in the range of 6.1×10^（-5）～1.22×10^（-3） mol·L^（-1） with correlation coefficient（r） 0.9967 under the optimal conditions.The proposed method was applied to the analysis of 4 food samples.Results：The consequent data are in consistency with the data obtained with GB/T 22388—2008 HPLC method,as proved by t - test.The RSD is less than 2.9%～4.0%（n =5）,recovery is in the range of 71.1%～85.5%.It is demonstrated that this method is simple and highly sensitive.
出处
《中国粮油学报》
EI
CAS
CSCD
北大核心
2010年第7期120-124,共5页
Journal of the Chinese Cereals and Oils Association
关键词
食品
三聚氰胺
茜素红
荷移反应
分光光度法
Melamine
Alizarine red
Charge - transfer reaction
Spectrophotometry
