摘要
采用分光光度法研究了头孢拉定与四氯苯醌的荷移反应。以正丙醇为介质，于pH9．00的硼砂缓冲液中，以十二烷基苯磺酸钠为增稳剂，四氯苯醌和头孢拉定形成1：1型稳定的荷移络合物，最大吸收波长在363nm处，线性范围为1．8×10^-6-2．0×10^-8mol／L，检出限为9．6×10^-9moL／L。方法用于药物制剂中头孢拉定含量的测定，平均回收率为98％。
The charge-transfer complex （ CTC ） of tetrachlorobenzo quinine with cefradine was studied in detail by spectrophotometry. It is found that in pH 9.00 borate buffer solution, cefradine can react with tetrachlorobenzo quinine to form 1 ： 1 complex in the presence of propanol solution, which gives a maximum absorption peak at 363 nm. Beer＇ s law is obeyed in the concentration range of 1.8 ×10^-6 -2. 0 ×10^-8mol/L. The detection limit is 9. 6 ×10^-9 mol/L. The method is simple ,rapid and sensitive and it can be used for the content determination of cefradine in various dosage forms. The average recovery is 98%, and the result is satisfactory.
出处
《分析试验室》
CAS
CSCD
北大核心
2014年第2期180-182,共3页
Chinese Journal of Analysis Laboratory
基金
陕西省重点实验室项目(12JS119)
延安大学国家级大学生创新训练计划项目(201210719008)资助
关键词
荷移反应
分光光度法
四氯苯醌
头孢拉定
十二烷基苯磺酸钠
Charge benzene sulphonate transfer reaction
Spectrophotometry
Tetrachlorobenquinone
Cefradine
Sodium dodecyl
