摘要
目的建立水中7种头孢抗生素(头孢羟氨苄、头孢氨苄、头孢哌酮、头孢呋辛、头孢拉定、头孢他定、头孢噻肟)的固相萃取(SPE)-高效液相色谱(HPLC)测定法。方法检测波长和参照波长分别为254和270nm,利用梯度淋洗程序,7种头孢抗生素在20min内完全分离。经过优化,水样预处理使用HLB柱进行SPE,调pH值至3,NaCl加入量为6.0g/L,进行HPLC测定。结果头孢呋辛、头孢噻肟、头孢他定的浓度在0.05～5.00mg/L范围内,头孢羟氨苄、头孢氨苄、头孢拉定的浓度在0.10～10.00mg/L范围内,头孢哌酮的浓度在0.20～20.00mg/L范围内,7种头孢抗生素的线性较好,均r>0.99,检出限为0.05～0.39μg/L。该方法在超纯水中的平均回收率为86.64%～105.28%,RSD为2.61%～11.64%。结论该方法的回收率较高,重复性好,准确性和灵敏度较高,适用于同时测定水中7种头孢抗生素。
Objective To develop a satisfactory and reliable method for simultaneous determination of seven cephalosporin antibiotics （cefadroxil, cephalexin, cefoperazone, cefuroxime, cefradine, cefiazidime and cefotaxime） in water samples using solidphase extraction and high performance liquid chromatography. Methods The detection and reference wavelengths were 254 and 270 nm,respectively. The analytes were separated within 20 min by a gradient elution program. The optimized pretreatment of water samples was as following： using hydrophilic-lipophilic balance HLB as SPE cartridges, adjusting pH to 3 and adding 3.0 g NaCl per 500 ml water sample. Results The linear range of the method was 0.05-5.00 mg/L for ceftazidime, cefotaxime and cefuroxime, 0. 10-10.00 mg/L for cefadroxil, cephalexin and cefradine, and 0.20-20.00mg/L for cefoperazone. The coefficients for the analytes were above 0.99 and the limits of determination were 0.05-0.39μg/L. The recoveries of seven cephalosporin antibiotics in purified water were 86.64%-105.28%, and its relative standard deviation were 2.61%-11.64%. Conclusion The method was sensitive, accurate and repeatable, it is applicable to the determination of the cephalosporin antibiotics in waters.
出处
《环境与健康杂志》
CAS
CSCD
北大核心
2009年第10期911-913,共3页
Journal of Environment and Health
基金
国家自然科学基金资助项目(50608050
30970531)
关键词
水
头孢抗生素
色谱法
液相
固相萃取
Water
Cephalosporin antibiotics
Chromatography, liquid
Solid phase extraction
