摘要
目的:观察Wistar大鼠、自发性高血压大鼠(spontaneously hypertensive rat,SHR)、2型糖尿病Goto-Kakizaki(GK)大鼠肾上腺类固醇及儿茶酚胺合成酶相关基因的表达特征。方法:以16周龄Wistar大鼠、SHR和GK大鼠为研究对象,采用大鼠四诊计量化诊法及GeneChip RatExon 1.0 ST Array等技术,检测正常与气虚Wistar大鼠、气虚和气盛SHR、气虚和气盛GK大鼠的肾上腺基因表达谱,分析类固醇及儿茶酚胺合成酶相关基因在不同证候大鼠肾上腺中的表达情况,以1.5倍筛选差异表达基因。结果:筛选获得31个基因在证候中存在差异表达。Hsd3b6在糖尿病气虚和气盛大鼠肾上腺组织的表达显著下调6.0倍,Cyp11b2在糖尿病气虚证大鼠肾上腺组织中的表达显著上调1.5倍以上。类固醇激素调节因子Por、Hsd11b2和Nr2f6基因在不同证候的表达均上调,Cyp2c23、Cyp4a3、Cyp4a8和Cyp2e1基因在不同证候的表达均下调,Srd5a1和Nr4a1基因仅在糖尿病大鼠肾上腺的表达上调,Lss仅在高血压病大鼠肾上腺的表达下调。儿茶酚胺合成酶Th在高血压气虚证、糖尿病气虚证与气盛证大鼠上调1.5倍以上,Ddc在糖尿病气盛证上调1.5倍以上,Dbh在糖尿病气虚证与气盛证上调达3.0倍。儿茶酚胺代谢酶Comt基因在糖尿病气盛证、气虚证下调1.5倍以上,Mao基因却在高血压气盛证、气虚证上调1.5倍以上。结论:SHR和GK大鼠类固醇激素以及儿茶酚胺合成途径中部分基因存在显著的差异表达特征,且可能与证候的发生有关。
Objective：To study the gene expression characteristics of steroid- and catecholamine-synthesizing enzymes in adrenal gland of spontaneously hypertensive rats（SHRs）,Goto-Kakizaki（GK） rats and normal Wistar rats with the same traditional Chinese medicine syndromes. Methods：Sixteen-week-old Wistar rats,SHRs and GK rats were used.By the quantitative four diagnosis and syndrome differentiation methods and GeneChip Mouse Exon 1.0 ST Array,we observed adrenal gland gene expressions in normal Wistar rats,qi deficiency Wistar rats,SHRs with qi deficiency and qi excess,GK rats with qi deficiency and qi excess.Differentially expressed genes of steroid- and catecholamine-synthesizing enzymes and their regulatory factors were analyzed. Results：Thirty-one genes were differentially expressed among all syndromes.Hsd3b6 was down-regulated significantly 6.0-fold in GK rats with qi deficiency syndrome and qi excess syndrome,and Cyp11b2 was up-regulated 1.5 times in GK rats with qi deficiency syndrome.Por,Hsd11b2,and Nr2f6 were up-regulated in all syndromes,and Cyp2c23,Cyp4a3,Cyp4a8 and Cyp2e1 were down-regulated.However,Srd5a1 and Nr4a1 were up-regulated only in GK rats,and Lss was down-regulated only in SHRs.Th was up-regulated 1.5 times in SHRs with qi deficiency syndrome,GK rats with qi deficiency syndrome and GK rats with qi excess syndrome.Ddc was up-regulated 1.5 times in GK rats with qi excess syndrome.Dbh was up-regulated 3.0 times in GK rats with qi deficiency syndrome and qi excess syndrome.However,Comt was down-regulated 1.5 times in GK rats with qi deficiency syndrome and qi excess syndrome,and Mao was up-regulated 1.5 times in SHRs with qi deficiency syndrome and qi excess syndrome. Conclusion：Some genes associated with steroid- and catecholamine-synthesizing pathways were differentially expressed in SHRs and GK rats,and the differentially expressed genes may be related to the development of traditional Chinese medicine syndromes.
出处
《中西医结合学报》
CAS
2010年第4期352-357,共6页
Journal of Chinese Integrative Medicine
基金
2006上海市教育委员会学科建设项目
