摘要
目的研究纤维蛋白原（fibrinogen，Fg）即链-854G／A、-455G／A、-249C／T、-148C／T、448G／A和Bcl-1G／A位点基因多态性与肥胖症的影响因素及血浆Fg含量和聚合功能表达的关系。方法选取开滦集团离退休职工1391人，依体重指数将其分为体重正常、超重及肥胖组，抽取静脉血测定血生化、血浆Fg浓度和纤维蛋白单体聚合反应速率（fibrin monomer polymerized velocity，FMPV）、最大光密度（Amax）、FMPV／Amax等反映Fg聚合功能的指标，应用聚合酶链反应一限制性片段长度多态技术进行即链6个位点多态性检测。结果Fg即6个位点中仅Bcl-1A等位基因和变异基因型在超重组的频率分布明显高于正常体重组（P〈0．01）；3组均为FgBβ-854变异基因型人群Fg浓度、FMPV、FMPV／Amax显著高于野生型（P〈0．01），超重组即-455变异基因型人群FMPV／Amax以及Bβ-249变异基因型人群FMPV分别高于野生型（P〈0．05）。结论Fg Bβ Bcl-1A等位基因及其变异基因型人群是易发体重超重的高危人群，Bβ—455和-249的变异基因型可通过调控血浆Fg聚合功能的表达而成为发生超重的累效基因。
Objective To study the association of fibrinogen（Fg） Bβ-854G/A, -455G/A, - 249C/T, -148C/T, 448G/A and Bcl-1G/A gene polyrnorphisms with factors affecting obesity, and the fibrinogen function such as plasma fibrinogen concentration and molecular reactivity. Methods One thousand and three hundred ninety-one subjects from Kailuan corporation were enrolled by medical examination and questionnaire survey, and were divided into normal weight, overweight and obese groups based on body mass index （BMI）. Blood biochemistry, fibrinogen concentration, fibrin monomer polymerized velocity （FMPV）, and FMPV/A were measured. The gene polymorphisms of the six loci were detected by polymerase chain reaction restriction fragment length polymorphism. Results The frequencies of Bcl-IA and its mutated genotype in the overweight group were significantly higher than that in the normal weight group （P〈0.01）. In all the three groups, Fg concentration, FMPV, FMPV/Amax in individuals with Bβ-854 mutated genotype were significantly higher than those with wild type genotype （P〈0.01）, and in the overweight group, FMPV/A in those with Bβ-455 mutated genotype, FMPV in those with Bβ-249 mutated genotype, were higher than those with wild type genotype （P〈0.05）. Conclusion Individuals with Bcl-IA and its mutated genotype are susceptible to overweight. The Bβ --455 and --249 mutated genotypes are accumulative genes for overweight by regulating the Fg function.
出处
《中华医学遗传学杂志》
CAS
CSCD
北大核心
2010年第2期198-203,共6页
Chinese Journal of Medical Genetics
