摘要
目的:研究血压对多态性血浆纤维蛋白原(Fg)Bβ链-854G/A、-455G/A、-249C/T、-148C/T、448G/A和Bc l-1G/A基因功能表达的影响。方法:选取开滦集团离退休职工1 391人进行体检和问卷调查,受检者清晨抽取静脉血标本测定血浆Fg浓度和纤维蛋白单体聚合反应速率(FMPV)、最大吸光度(Am ax)、FMPV/Am ax等反映Fg聚合功能的指标,然后立即测定血压;应用聚合酶链反应-限制性片段长度多态性技术(PCR-RFLP)检测Fg Bβ链6个多态性位点的基因型。结果:高血压状态组(HG)与正常血压组(NG)组间6个基因多态性位点等位基因及各基因型的频率分布均无显著差异(P>0.05)。HG与NG的Fg浓度、FMPV/Am ax无显著差异(P>0.05),但HG的FMPV、Am ax低于NG(P<0.05),HG和NG的Bβ-854位点变异基因型组Fg浓度、FMPV、Am ax、FMPV/Am ax均高于野生型(P<0.05),且NG的Bc l-1位点变异基因型的Fg浓度、FMPV高于野生型(P<0.05),但HG的Bβ-854和Bc l-1变异基因型组的Fg、FMPV、Am ax均低于NG(P<0.05)。结论:Fg Bβ-854基因位点是调控Fg功能表达的重要部位,Bc l-1的变异基因型可使血浆Fg浓度和FMPV升高;高血压状态可以明显抑制这种调控作用,并损伤纤维蛋白单体聚合成二聚体的过程,从而导致特殊类型的凝血功能障碍。
AIM：To study the correlation between blood pressure and 6 common fibrinogen（Fg） Bβ-chain gene polymorphisms（Bβ-854G/A,-455G/A,-249C/T,-148C/T,448G/A,Bcl-1G/A）,and to investigate the effect of blood pressure on plasma fibrinogen concentration and the functional expression of fibrinogen.METHODS： 1 391 subjects from Kailuan Group Corporation were enrolled for medical examination and questionnaire survey.Venous blood were collected in the early morning to measure Fg concentration,fibrin monomer polymerized velocity（FMPV）,absorbance maximum（Amax） and FMPV/Amax.The blood pressure was also measured and the subjects were accordingly divided into hypertension group（HG） and the normal blood pressure group（NG）.The 6 gene polymorphisms were detected by polymerase chain reaction-restriction fragment length polymorphism（PCR-RFLP）.RESULTS： No differences of the genotype frequencies of the 6 sites between hypertension group（HG） and normal blood pressure group（NG） were found（P0.05）.No differences of Fg concentration and FMPV/Amax between HG group and NG group were observed（P0.05）.However,FMPV and Amax in HG group were lower than those in NG group（P0.05）.In HG and NG group,the Fg concentration,FMPV,Amax,FMPV/Amax in the subjects with Bβ-854 mutated genotype were all higher than those in the subjects with wild genotype（P0.05）.In NG group,the Fg concentration and FMPV in the subjects with Bβ Bcl-1 mutated genotype were higher than those in the subjects with wild genotype（P0.05）.Fg,FMPV and Amax in the subjects with Bβ-854 and Bcl-1 mutated genotype in HG group were lower than those in NG group（P0.05）.CONCLUSION： Fg Bβ-854 is one of the main gene situs of regulating the functional expression of fibrinogen.Bcl-1 mutated genotype increases plasma Fg concentration and FMPV.Hypertension decreases the effect of this regulation,and affects the process of fibrin monomer aggregation into dimer,resulting in a special type of coagulation dysfunction.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2010年第12期2327-2331,共5页
Chinese Journal of Pathophysiology
