摘要
目的通过分析不同阶段HIV感染者外周血CD4^＋CD25^hi调节性T细胞（CD4^＋CD25^hi regulatory T cells，Treg cells）与外周血免疫状态和病毒载量的相关性，探讨Treg细胞对HIV／AIDS发病进程的影响。方法采集116例HIV感染者和21例正常人对照外周血，用4色流式细胞术进行CD4^＋和CD8^＋T细胞绝对数计数；用3色流式细胞术进行Treg细胞测定；用荧光定量PCR法进行HIVRNA载量测定。实验数据用回归统计学方法和T检验方法进行分析。结果HIV感染者外周血Treg细胞频率在HIV感染初期显著下降，之后随着疾病的进程逐渐升高。在CD4^＋T细胞大于300／μl的患者低于正常对照组，在CD4^＋T细胞小于100/μm的患者高于正常对照组，差异具有统计学意义。Treg细胞频率与CD4^＋T淋巴细胞绝对数和CD4v／CD8^＋之间均呈负相关，其相关系数r和P值各为r=-0．564，P〈0．001和r=-0．377，P〈0．001；Treg细胞频率与血浆HIV病毒载量呈正相关，其相关系数r=0．514，P〈0．001。结论CD4^＋CD25^hi Treg细胞可能是参与艾滋病免疫发病机理的重要细胞，在HIV感染发病进程的不同阶段具有不同的意义，其确切机制有待进行进一步研究。
Objective Analyzing the relationships between peripheral blood CD4^＋ CD25^hi regulatory T （Treg） cells and peripheral blood immune status or plasma HIV-lviral load in HIV-infected individuals, so as to determine whether Treg were related to the progression of HIV-infected disease. Methods 116 HIV-infected patients in different stages and 21 healthy control individuals were included in this study. The CD4^＋ and CD8^＋ T cell counts were determined by a standard 4-color flow cytometry technique. The Treg cells were examined with 3- color immune staining flow cytometry. The plasma HIV-1 viral load was detected by real time PCR. Results The frequencies of Treg cells decreased in HIV-infected individuals with high CD4^＋ T cell counts（ 〉 300/μl） compared with normal controls. With the progression of disease the frequencies of Treg cells were raised gradually, until were increased in HIV-infected individuals with low levels of CD4^＋T cell counts （ 〈 100/μl）. In addition, the frequencies of Treg cells were inversely related to CD4^＋T cell counts and CD4^＋/CD8^＋ ratio, data showed a statistically significant （respectively, r = - 0.564, P 〈 0.001; r = - 0.377, P 〈 0.001）. Furthermore, the proportions of Treg cells were closely related to plasma HIV-1 RNA viral load （ r = 0.514, P 〈 0.001 ）. Conclusion CD4^＋ CD25^hi Treg cells should be a kind of important cells participating the immunopathogenesis of AIDS. It may play different roles in different stages of HIV-infected disease. The exact mechanism of Treg cells in the progression of the HIV-infected disease needs to be investigate further.
出处
《中华实验和临床病毒学杂志》
CAS
CSCD
北大核心
2009年第5期361-363,共3页
Chinese Journal of Experimental and Clinical Virology
基金
北京市自然科学基金（No.7042028）
北京市留学人员科技活动项目择优资助经费资助
