摘要
目的对中国HIV-1感染者CD4^＋CD25^＋Foxp3^＋调节性T细胞水平及与疾病进展相关性进行研究，探讨CD4^＋CD25^＋Foxp3^＋调节性T细胞在HIV-1疾病进程中发挥的作用。方法选取35名HIV-1感染者及14名健康对照，应用流式细胞仪胞内染色技术在单细胞水平检测CD4^＋CD25^＋Foxq3^＋调节性T细胞表达及淋巴细胞活化、凋亡水平。结果中国HIV-1感染者CD4^＋CD25^＋Foxp3^＋T细胞水平与CD4^＋T细胞显著负相关（r=-0．544，P=0．001），与病毒载量明显正相关（r=0．484，P=0．026），与CD4^＋T细胞凋亡（CD95表达）水平明显正相关（r=0．431，P=0．011）。艾滋病人CD4^＋CD25^＋Foxp3^＋T细胞水平明显高于无症状HIV感染者（P〈0．05），与健康人相比差异无统计学意义。艾滋病人CD4^＋、CD8^＋T细胞凋亡水平明显高于无症状HIV感染者及健康人（P〈0．05），艾滋病人及无症状HIV感染者CIM^＋、CD8^＋T细胞活化明显高于健康人（P〈0．05）。结论中国HIV-1感染者CD4^＋CD25^＋Foxp3^＋调节性T细胞水平与疾病进展明显相关。
Objective To detect the frequency of CD4^＋ CIT25^＋ Foxp3^＋ regulatory T cells of H1V-1 infectecl individuals in China, and to study its association with disease progression. Methods Thirty-five untreated H1V-1 infected patients and 14 normal controls were enrolled and the frequency of CD4^＋ CD25^＋ Foxp3^＋ regulatory T ^＋ was tested in one cell levd by flow cytometry. To study the correlation between the frequency of CD4 ^＋ CD25^＋ Foxp3^＋ regulatory T cells and disease prngression, the absolute CD4^＋ T cells, viral load, apoptosis and activation of T cells were also examined. Retails It was found that CD4 ＋ CD25 ＋ Foxp3 ＋ regulatory T cells number is strongly conelated with absolute CD4 count （r = -0.544, P=0.001）, viral load （r =0.484, P = 0.026） and CD4^＋ CD95^＋ T cells （r=0.431, P=0.011）. CD4^＋ CD25^＋ Foxp3^＋ T cell number is higher in AI^＋ patiets than in asymptomafic HIV-1 infected patients （ P 〈 0.05）. The CD95 e^＋ression on CD4^＋ , CD8^＋ T cells in AIDS patients was higher than that in asymptomafic H1V-1 infected patients and normal controls （ P 〈 0.05）. The activation of CD4^＋ , CD8^＋ T cells in AIDS patients and asymptommatic H1V-1 infected patients was higher than that innormalcontrols（P〈0.05）. Conclusion Thefrequency of CD4^＋CD25^＋Foxp3^＋ regulatoryTcelisinHIV-1 infected patients of China significantly correlated with disease progression.
出处
《中华微生物学和免疫学杂志》
CAS
CSCD
北大核心
2007年第3期278-281,共4页
Chinese Journal of Microbiology and Immunology
基金
国家自然科学基金（30600532）
国家重点基础研究发展973计划（2006CB504206）
辽宁省医学创新工程项目（辽卫函字[2004]37）
