摘要
目的探讨长期丘脑底核脑深部电刺激对猴帕金森病模型纹状体区细胞外液中γ-氨基丁酸含量的影响。方法建立偏侧猴帕金森病模型,在患侧丘脑底核植入人用脑深部刺激电极,在双侧壳核、尾状核头部植入微透析探针,同期猴背部皮下植入脉冲发生器,通过皮下导线与刺激电极相连。通过测试后给予有效高频电刺激,分别在打开脉冲发生器前和开机后的不同时间点应用持续微透析的方法对纹状体细胞外液进行取样(开机后1周、1、8、12个月)。应用高效液相色谱荧光法检测开机前后的尾状核和壳核细胞外液的γ-氨基丁酸的含量变化。结果在丘脑底核慢性高频电刺激下,电极侧尾状核及壳核的γ-氨基丁酸含量在开机后1周、1个月时均较开机前明显增加(P<0.05),但在开机后8、12个月时γ-氨基丁酸含量较开机前、开机1周、1个月时又明显下降(P<0.05)。结论丘脑底核脑深部刺激后可引起纹状体区细胞外液中γ-氨基丁酸的含量短期内明显升高,但长期的刺激后其含量会明显下降。这可能是丘脑底核脑深部电刺激的作用机制之一。
Objective To investigate the influence of high fequency stimulation of the subthalamic nucleus（STN-DBS）on the level of γ-aminobutyric acid（GABA）in corpus striatum of hemiparkinsonian monkeys.Methods The leads of deep brain stimulation were implanted in subthalamic nucleus of hemiparkinsonian monkes,the pulse generators were implanted subcutaeous in back of monkeys at the same time,the lead and the pulse generator connect by the connection wire.All monkeys were implanted microdialysis cannulae to bilateral putamen and caudate nucleus.Collecting the dialyate before turning on the pulse generator,and at 1 week,1,8 and 1 2 months after high frequency stimulation.The level of GABA were determined by high performance liquid chromatography and fluorescence detection（HPLC-FD）.Results After high frequency stimulation,PD symptoms of monkeys significantly improved.The levels of GABA in putamen and caudate nucleus of electrodes side at 1 week,1 month increased significantly compared with before turning on the pulse generator while decreased at 8,1 2 months.Conclusion STN-DBS can increased the level of GABA in the striatum extracellular fluid significantly in the short term,but long-term stimulation decreased its level.This maybe play a role in the mechanism of STN-DBS.
出处
《立体定向和功能性神经外科杂志》
2009年第5期261-264,共4页
Chinese Journal of Stereotactic and Functional Neurosurgery
基金
国家自然科学基金项目(编号:30471776)
关键词
丘脑底核
脑深部电刺激
微透析
γ-氨基丁酸
纹状体
Subthalamic nucleus
Deep brain stimulation
Microdialysis
γ-aminobutyric acid
Corpus striatum
