摘要
目的探索脑脊液诱导人间充质干细胞定向分化为神经干细胞的可行性。方法分别采用同种异体脑脊液和细胞生长因子为诱导剂，体外诱导人脐血源和骨髓源间充质干细胞分化为神经干细胞。从形态学、免疫组化、荧光免疫组化法、反转录聚合酶链反应（RT-PcR）方法对诱导后细胞进行鉴定。结果两种方法诱导后间充质干细胞呈现神经干细胞改变，均可分化为神经元、星形胶质细胞和少突胶质细胞，并表现相应的特征形态结构、表型和生物学特性，细胞长势良好。而脑脊液诱导组，从形态上与细胞生长因子诱导组基本一致，但从时间上缩短神经干细胞、神经样细胞的生长周期。结论两种诱导方法均可在体外定向诱导不同来源人间充质干细胞转化为神经样细胞，而脑脊液诱导组诱导时间和细胞周期均较短。
Objective To investigate the feasibility of Cerebrospinal fluid induces human mesenchymal stem cells （MSCs） directed to differentiation into neural stem cells. Methods MSCs derived from adult bone marrow and new born cord blood were isolated and cultivated in vitro. MSCs were induced and differentiated by cerebrospinal fluid and growth factor, respectively. Morphological changes of induced cells were observed. The expression of cell surface antigens was detected by flow cytometer, immunocytochemistry and RT-PCR. Results MSCs were induced and differentiated into neuron like cells by cerebrospinal fluid and growth factor respectively. The induced cells expressed NSE, NF after induction. There were no significant differences between two groups in morphological changes. However, the growth cycle of neural stem cells and Neuron-like cells was shortened in the group induced by cerebrospinal fluid. Conclusion MSCs consecutively treated with cerebrospinal fluid and growth factor is induced to neuron-like cells, and the cell cycle and time induced by cerebrospinal fluid get shorter.
出处
《国际麻醉学与复苏杂志》
CAS
2009年第5期394-398,共5页
International Journal of Anesthesiology and Resuscitation
关键词
脑脊液
间充质干细胞
神经样细胞
诱导分化
Cerebrospinal fluid
MSCs
Neuron-like cells
Induced differentiation
