摘要
目的 体外获取高浓度神经干细胞 (NSC)。方法 加入bFGF培养E12天大鼠胚脑的全部细胞 ,用荧光免疫组化法显示。结果 加bFGF的神经干细胞 (Neuralstemcell,NSC)大量分裂 ,形成大量的细胞分裂球 ,传到 4代时 ,90 %以上的细胞表达nestin。对照组分裂的细胞少 ,维持到第二代细胞逐渐死亡。结论 bFGF对胚胎E12天大鼠脑细胞的NSC有明显的促分裂作用 。
Objective To gain high purified neural stem cells.Methods The whole brain from embryonic day 12 of rats were cultured in the present of bFGF and detected by immunofluorescence staining.Results The neural stem cells (NSC) formed a lot cellular spheres in the present of bFGF till 4 genenations.The majority of the cells,about more than 90%express Nestin.The proliferation of the NSCs in the control group appeared for a short time.Conclusion bFGF obviously promoted the proliferation of NSCs from E12 rats.The high-purified NSCs could gain by culturing the brain cells form younger embryonic rats in the present of bFGF.
出处
《四川解剖学杂志》
2002年第3期140-141,共2页
Sichuan Journal of Anatomy
