摘要
以小鼠脾淋巴细胞为实验材料,采用MTT微量自动比色法判断细胞分裂状况,观察PHA的促淋巴细胞分裂作用时Ca^(2+)的影响。结果发现:加Ca^(2+)的最大刺激组的光密度(OD)值比对照组提高了35.4％,Ca^(2+)的最大刺激浓度为4％葡萄糖酸钙.结果表明Ca^(2+)有增强PHA的有丝分裂原作用,并且存在明显的量—效关系。
The experimential material in this paper is the lymphocyte from mice spleen, the micro-MTT auto chromatometry was adopted to judge the status of Mitosis, the purpose is to observe effect of Ca2+ on phytohemagglutinin (PHA) -stimulated lymphocytes mitosis, It was found that the OD value of the group of largest effect (with Ca2+) increased by 35. 4% compared with the control group and the concentration of Ca2+ achieving the largest effect was 4 % (calium glucuromide). The results showed that Ca2+ could enhance the mitogenic effect of PHA and displayed a marked concentration-effect relationship.
出处
《咸宁医学院学报》
1995年第1期4-7,共4页
Journal of Xianning Medical College
基金
CMB资助课题
