摘要
试验对植物血凝素在艾维茵雏鸡新城疫疫苗免疫过程中的效果进行了研究。经过雏鸡新城疫母源抗体水平的检测后,选择抗体滴度约为2.5log2的雏鸡90羽,随机分成3组,分别为溶剂对照组、单免组和PHA组。单免组和PHA组分别于7,21d接种Ⅳ系LaSota弱毒疫苗,PHA组还同时肌肉注射植物血凝素0.01mL/只。各组鸡于第1次免疫后的14,21,28,35,42,49和56d测定血液中鸡新城疫特异性抗体水平。结果显示,经2次免疫后PHA组鸡的抗体滴度比单免组和对照组鸡的高,差异显著(P<0.05),说明PHA能显著增强雏鸡对LaSota弱毒苗的免疫反应。
The effect of phytohemagglutinin on the immune efficacy of Newcastle disease vaccine was studied with Avian chickens as experimental animals. After the maternal antibody level was detected, 90 chickens with antibody level of 2.5 log2 were selected and randomly divided into three groups, that is, the control group, the single immune group, and the PHA group. The chickens of the single immune group and the PHA group were immunized with LaSota attenuated vaccine, when they were 7-day-old and 21-day-old. And each chicken of the PHA group were administrated 0.01 ml PHA via intramuscular injection. The titers of antibodies against NDV were respectively determined at 14, 21, 28, 35, 42, 49, and 56 d post the first inoculation. The results showed that the HI antibody titers against NDV were higher in PHA group than in another two groups with significant difference （P〈0.05）, indicating PHA may greatly strengthen the immune efficacy of LaSota attenuated vaccine on chickens.
出处
《畜牧与饲料科学》
2009年第5期130-132,共3页
Animal Husbandry and Feed Science
