摘要
该项研究用3mKCl提取胃癌抗原（GCaAg），免疫小鼠制备特异性转移因子（STF），应用巨噬细胞（MΦ）移动抑制试验（MIT），测得GCaAg致敏动物淋巴细胞加GCaAg和加STF＋GCaAg试验组，MΦ移动面积明显小于对照组（P＜0．001和P＜0．01）。移动指数（MI）在0．8以下，均为阳性。在MTT比色中加GCaAg和加STF组OD值明显大于空白对照组（P＜0．05和P＜0．001）；淋转指数STF组最高（1．73）。两项试验提示STF确有激活淋巴细胞的作用，并显示抗原依赖的特异性活性。在MIT中显示STF作用无种属特异性限制。这一点为动物来源的STF治疗人类肿瘤提供了依据。
In this study, we extracted Gastric Cancer Antigen (GCaAg) with 3MKCl, immunized mice and prepared specific tramsfer Factor (STF). WithM Migration inhibitory test (MIT ). i, e. mouse lymphocytes sensitized by GCaAg adding only GCaAg and adding both STF and GCaAg were done. During the Test, the testing group's Mrp migration area is obvlously smaller than that of contrasting group's area. (P＜0.001 and P＜0.01). The migration Index (MI) are all positive under 0. 8. During MTT Colorimetry, the Values of OD adding GCaAg groups and adding STF groups were obviously larger than that of contrasting groups (P＜0. 05and P＜0.001 ). The lymphocyte transformation lndex of STF groups are the highest (1. 73). The both tests suggest that STF did have the effect of ativating lympho cyte and show that Ag dependent specific activation. From MIT test, show that STF effect has no species limit, and this provides the basis for treat human tumor with STF which came from animals.
出处
《肿瘤防治研究》
CAS
CSCD
北大核心
1997年第6期361-364,共4页
Cancer Research on Prevention and Treatment
关键词
胃肿瘤
胃癌抗原
STF
MTT
特异性转移因子
Gastric cancer Antigen
Gastric cancer specific transfer factor
Macrophage Migration Inhibitory test
MTT Colorimetry.
