摘要
应用单克隆抗体，流式细胞仪等方法，观察雷公藤多甙片水溶性及醇溶性成分对外周血淋巴细胞的作用。结果表明，雷公藤多甙片中水溶性成分及醇溶性成分在体外，对人外周血ＣＤ＿４、ＣＤ＿８的作用一致，使ＣＤ＿４、ＣＤ＿８阳性细胞增多（Ｐ＜０．０５），且二者都能增强ＰＨＡ对ＣＤ＿（２５）表达的诱导作用（Ｐ＜０．０５），雷公藤多甙醇溶性成分为２００μｇ／ｍｌ对静止期淋巴细胞有促分裂作用，而当其浓度达１ｍｇ／ｍｌ时，能抑制ＰＨＡ对静止期淋巴细胞的促分裂作用。本文结果提示：雷公藤多甙中的水溶性成分在体外对淋巴细胞有免疫增强作用，而醇溶性成分在小剂量时有免疫促进作用，但大剂量时则为免疫抑制作用。
Abstract Monollonal antibody and flow cytometry were used to observe the effects of wat er-soluble and ethanol-soluble components in tabellae glucosidorum tripterygiitotorum(TGT)on peripheral blood lymphocytes(PBL).Results showed that there were similar effects of the 2 compo nents in TGT to PBL,the CD_4 and CD_8 positive cells all increased(P<0. 05).Meanwhile,both components enhanced the PHA-induction of CD_(25) expression. The ethanol component of TGT at 200 μg/ml had mitogenic effect on static phase lymphocytes. At 1mg/ml, it can inhibit the PHA mitogenesis effect on static lymphocytes. Our results showed that the water-soluble component of TGT has an immuno-enhancing effect on lymphoeytes in vitro,while the ethanol-soluble compo nent has an immuno-promoting effect at a low dose and has an immuno-suppressine effect at higher doses.Keywrods: tabellae glucosidorum tropterygiitotorum peripheral-blood lymphocyte
出处
《上海交通大学学报（医学版）》
CAS
CSCD
1994年第S1期57-61,共5页
Journal of Shanghai Jiao tong University:Medical Science
