摘要
褐变是蝴蝶兰离体培养中的常见现象,试验尝试探寻蝴蝶兰花梗腋芽离体快繁过程中有效控制其褐变的方法,诱导丛生芽。试验证明:带腋芽花梗节段褐变程度比腋芽要轻;适当的暗培养有利于减轻外植体的褐变;细胞分裂素KT比BA的褐变症状要轻,但丛生芽的诱导率不如BA;适当添加一定剂量的PVP、活性炭、柠檬酸等可减轻褐变,柠檬酸、PVP效果比活性炭显著,为特色花卉的工厂化生产提供参考。
Browning is a common phenomenon of Phalaenopsis in vitro culture, this test attempts to explore the rapid propagation effective way of butterfly orchid stems Axillary process of in vitro to control their browning, bud induction. Tests prove that all those with axillary pedicel browning segment are lighter than axillary and Cytokinin KT browning symptoms lighter than BA, Bud induction rate than BA; It may reduce the browning that add the appropriate dose of PVP, activated carbon, citric acid etc, citric acid and PVP significantly make better than activated carbon, which provides a reference for the factory about the production of Characteristics flowers and plants.
出处
《中国农学通报》
CSCD
北大核心
2009年第9期192-195,共4页
Chinese Agricultural Science Bulletin
关键词
蝴蝶兰
花梗腋芽
离体快繁
褐变
phalaenopsis, pedicel axillary, tissue culture, browning
