摘要
目的测定盐酸洛美沙星（ｌｏｍｅｆｌｏｘａｃｉｎ，ＬＭＬＸ）局部用药后兔眼组织的浓度。方法将１５只兔分为５组。均局部应用ＬＭＬＸ眼液，Ⅰ组为单次滴眼，Ⅱ组为多次滴眼，Ⅲ组为去除角膜上皮后单次滴眼，Ⅳ组（色素兔）单次滴眼，Ⅴ组为结膜下一次性注射（０５ｍｇ）。各组均于用药３０分钟后，抽取房水，摘除眼球，全部标本均采用高效液相色谱法测定眼组织中ＬＭＬＸ的浓度。结果Ⅰ组单次滴眼３０分钟后，角膜、房水及虹膜睫状体组织的药物浓度分别为８７５±３０９μｇ／ｇ、０２１±０１μｇ／ｍｌ及１４９±０３９μｇ／ｇ。其他各组的药物浓度均明显高于Ⅰ组（Ｐ＜００１）。Ⅳ组色素兔中，眼色素组织有较高的药物浓度。结论兔眼局部应用ＬＭＬＸ后，眼内药物浓度超出多数病菌的最小抑菌浓度（ｍｉｎｉｍｕｍｉｎｈｉｂｉｔｏｒｙｃｏｎｃｅｎｔｒａｔｉｏｎｆｏｒ９０％ＭＩＣ９０），ＬＭＬＸ与色素组织有较好的亲和力。
Objective To measure the rabbit ocular tissue concentration of lomefloxacin (LMLX) after its topical application.Methods Fifteen rabbits (30 eyes) for the experiment (12 white rabbits and 3 pigmented rabbits) were divided into 5 groups. In the 1st group, 0.3% LMLX eyedrops were instilled once (1 drop each) in the 3 white rabbit′s eyes. In the 2nd group, 0.3% LMLX eyedrops were instilled for 6 times (1 drop once) with an interval of 5 minutes in the 3 white rabbit′s eyes. In the 3rd group, 0.3% LMLX eyedrops were instilled once (1drop each) in the 3 white rabbit′s eyes after removal of corneal epithelium. In the 4th group, 0 3% LMLX eyedrops were instilled once (1 drop each) in the 3 pigmented rabbit′s eyes. In the 5th group, LMLX 0.5 mg was subconjunctivally injected in the 3 white rabbit′s eyes. After the administration for 30 minutes, aqueous humor was collected and the eyeball was enucleated. All measurements were performed by high pressure liquid chromatography (HPLC).Results In the 1st group, the mean levels of drug were 8.75±3.09 μg/g in cornea, 0.21±.0.1 μg/ml in aqueous humor, and 1.49±0.39 μg/g in iris, respectively. The ocular tissue concentrations of the other groups were significantly higher than that of the 1st group. The pigmented tissue of pigmented rabbit′s eyes had relatively high concentration of LMLX.Conclusion All concentrations of LMLX in rabbit′s eyes are above the minimun inhibitory concentration for 90% (MIC 90 ) of most bacterial pathogens. LMLX has greater affinity in the pigmented tissue. These results show that LMLX may be an effective antimicrobial agent for prophylactic use in ophthalmic surgery and also for the treatment of intraocular infections due to susceptible organisms.
出处
《中华眼科杂志》
CAS
CSCD
北大核心
1998年第3期211-214,共4页
Chinese Journal of Ophthalmology
