摘要
以上海崇明养殖场堆肥为筛选材料，通过刚果红染色鉴定，获得一株高活力纤维素酶生产细菌。研究表明该菌为芽孢杆菌，适宜在pH值7．0～8．0、37～42℃条件下生长。该菌的CMC酶活力的最适pH值8．0，酶反应的最适温度50℃，酶活力最大时可达68．6U／ml。Ca^2＋、Cu^2＋、Co^2＋是纤维素酶的促进剂，高浓度的Cd^2＋和Pb^2＋是纤维素酶的抑制剂，并且该纤维素酶对乙醇热耐受能力较强。
A Bacillus strain was isolated from muck of Chongming which had high endo-β-glucanase producing ability proved by the Congo red dying method on culture plates. Optimal growing conditions of the strain were 37-42℃, pH value 7.0-8.0. It showed that the optimal pH value and temperature of cellulase activity （CMCase） were pH 8.0 and 50℃, and the maximal activity of CMCase is 68. 6 U/ml. In addition, Ca^2＋ , Cu^2 ＋and Co^2＋were the activators, Cd^2＋. and Pb^2＋ were the inhibitors of the enzyme. The cellulase was insensitive to ethanol.
出处
《药物生物技术》
CAS
CSCD
2009年第1期43-45,共3页
Pharmaceutical Biotechnology
基金
上海市引进技术的吸收与创新计划资助(06NY-122)
2007年度上海大学创新基金项目
关键词
筛选
沼气池
纤维素酶
细菌
酶活力
Screening, Biogas pool, Cellulose, Bacteria, Enzyme activity
