摘要
通过筛选获得高产纤维素酶细菌菌株,为纤维素酶制剂研制及纤维素酶工程菌构建提供试验材料。采用刚果红平板从牛羊粪便及腐败的玉米秸秆和木屑中分离产纤维素酶菌株,以DNS法测定酶活,根据酶活复筛产纤维素酶分离菌株;观察分离菌株的形态、染色与培养特性;应用16S rDNA通用引物扩增菌株基因组DNA,测序结果提交GenBank数据库进行Blast比对分析和相似性搜索,作出复筛菌株种的鉴定。结果表明:分离获得16株纤维素酶产量较高的细菌菌株,均为革兰氏阳性菌,菌体呈短杆状、具中央芽孢,经16S rDNA序列比对分析和相似性搜索,鉴定为10株枯草芽孢杆菌(Bacillus subtilis)、6株解淀粉芽孢杆菌(Bacillus amyloliquefaciens)。
In order to provide bacteria for development of high-yield cellulase bacteria strains were screened. sheep dung and putrid maize through the determination of teristics. 16S rDNA universal straws or wood chips Sepa with rate cellulase preparations and cellulase engineering,the cellulase producing strains isolated from cow or Congo red plate to screen cellulase producing strains enzyme activity with DNS assay, and observe its shape, dyeing and cultural charac- primers were species of rescreening strains. The result ind applie icated d to amplify genomic DNA of the strain, and to identify the that the isolated 16 bacterial strains with higher cellulase pro- duction were gram positive, short rod and central spores. In which, 10 strains were Bacillus subtilis, 6 strain were Bacillus am ylolique f aciens.
出处
《草原与草坪》
CAS
CSCD
2017年第2期7-11,19,共6页
Grassland and Turf
基金
甘肃省科技支撑计划项目(1204NKCA103)
甘肃省农业生物技术研究与应用开发项目(GNSW.2012-25)
甘肃农业大学盛彤笙科技创新基金项目(GSAU-STS-1232)资助
