摘要
从黄海边土壤中筛选到具有广谱抑菌活性白色粘细菌So ce cpu-1,次级代谢物活性组分在210nm紫外吸收值最大。研究了不同培养条件对次级代谢产物的产量影响。结果表明,So ce cpu-1在含10％w／v D312中性吸附树脂的M7培养基中,通氧量为总体积的70％,添加该粘细菌次级代谢产物的粗提物5μL作为诱导物,30℃,200r／min,培养6d,次级代谢产物产量可达到最大值。
A myxobacterial strain, So ce cpu-1, was isolated from a soil sample near the Huanghai beach. So ce cpu-1 had broad antimicrobial activity, active componcnt has maximal absorption at 210 nm. The effects of different culture conditions on thc yicld of thc secondary mctabolites were investigated, Thc results showed that, when cultivating the strain in thc M7 mcdium （containing 10% w/v D312 neutral absorber resin ）, the air up to 70% of the whole flask volume, adding 5μ L thc secondary mctabolites as the revulsant, at 30℃, 200f/min, for 6 days, the yield of the secondary metabolites achieved the maximum.
出处
《微生物学通报》
CAS
CSCD
北大核心
2006年第3期42-46,共5页
Microbiology China
基金
江苏省博士后基金资助
关键词
粘细菌
次级代谢产物
抑菌
培养条件
产量
Myxobacterium, Secondary metabolites, Antimicrobial activity, Culture conditions, Yield
