摘要
目的考察乌药叶总黄酮(LA-flavonoids)的抗氧化作用,初步探讨其抗氧化机制。方法在化学模拟体系中,利用总抗氧化能力和抗超氧阴离子自由基试剂盒评价LA-flavonoids抗氧化活性。利用腹腔注射四氯化碳(CCl4)造成小鼠急性肝损伤动物模型,以血清转氨酶活性以及总抗氧化能力、超氧化物歧化酶(SOD)、丙二醛(MDA)水平为指标判断LA-flavonoids对化学性肝损伤的保护作用,并通过逆转录-多聚酶链反应(RT-PCR)方法测定肝组织中相关抗氧化基因表达量的变化。结果在化学模拟体系中,随着LA-flavonoids浓度的增加,其总抗氧化活性和抗超氧阴离子自由基活性均显著增加。在CCl4肝损伤模型中,50～200mg/kg的LA-flavonoids均可显著降低肝损伤小鼠血清转氨酶的活性,显著增强总抗氧化能力和SOD活力,减少MDA释放;进一步研究发现,LA-flavonoids可显著提高受损肝组织中抗氧化相关基因thioredoxin(抗细菌硫氧还原蛋白)、hemeoxygenase-1(血红素加氧酶-1)以及peroxiredoxin-1(过氧化物酶-1)的表达。结论LA-flavonoids能通过清除自由基,抑制脂质过氧化产生,从而保护CCl4所致小鼠急性肝损伤,其机制可能与调节抗氧化相关基因的表达有关。
Objective To explore the anti - oxidation effect and mechanism of the total flavones from the leaf of Lindera aggregata （LA - flavonoids）. Methods The total antioxidation and anti - superoxide anion capacities were measured to evaluate the anti -oxidation activity of LA -flavonoids in the chemical model system in vitro. Acute liver injury mice model was induced with carbon tetrachloride （CCl4）, and the effect of LA - flavonoids on serum alanine aminotransferase （ALT） and aspartate aminotransferase （AST） levels as well as the contents of malondialdehyde （MDA）, superoxide dismutase （SOD） and total anti - oxidation capacity were examined. RT - PCR was used for the determination of oxidation - related genes expression in liver tissues of mice with liver injury. Results In the chemical model system in vitro, with the increase of LA - flavonoids concentration, the total anti - oxidation and anti - superoxide anion capacities were increased. In mice with CCh - induced acute liver injury, LA - flavonoids at the concentrations of 50 - 200 mg/kg significantly decreased the ALT and AST activities and MDA content, and increased the activities of SOD and total antioxidation capacity in serum, The mRNA expression of thioredoxin, heme oxygenase - 1 and peroxiredoxin - 1 in liver tissues were also increased by LA- flavonoids. Conclusion LA- flavonoids has protective effect against CCl4- induced acute liver injury by clearing away free radicals and inhibiting lipid peroxidation. Its antioxidant activity may be related to the regulation of some antioxidant - related genes.
出处
《中药新药与临床药理》
CAS
CSCD
2008年第6期447-450,共4页
Traditional Chinese Drug Research and Clinical Pharmacology
基金
江苏省六大人才高峰资助项目(A)。
关键词
乌药叶
总黄酮
抗氧化
四氯化碳
肝损伤
Leaf of Lindera aggregata （Sims） Kosterm
Total flavonoids
Anti - oxidation
Carbon tetrachloride
Liver injury
