摘要
为探讨金樱子多糖对CCl4所致小鼠急性肝损伤的保护作用。建立CCl4诱导的小鼠急性肝损伤模型,分光光度法检测丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)、碱性磷酸酶(ALP)、丙二醛(MDA)和还原型谷胱甘肽(GSH)含量,以及超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH2Px)的活性。结果表明:高剂量的金樱子多糖可显著降低CCl4所致急性肝损伤小鼠的肝脏指数(P<0.05)和脾脏指数(P<0.01);中、高剂量的金樱子多糖可显著降低CCl4所致急性肝损伤小鼠血清ALT、AST、ALP活性(P<0.01)和肝组织MDA含量(P<0.01),提高肝组织ALT、AST、ALP活性(P<0.01)和GSH-Px、SOD活性(P<0.01或P<0.05),并增加GSH的含量(P<0.01)。金樱子多糖对CCl4所致小鼠急性肝损伤具有一定的保护作用。
To observe the hepatoprotective effects of Rosa laevigata Michx. Polysaecharide （RLP） on acute hepatic injury mice induced by Carbon Tetraehloride （CCL）. The acute hepatic injury mice of model were induced by CCk. The levels of Alkaline Phosphatase （ALP）, Aspartate Transminase （AST）, Aalanine Aminotransferase （ALT）, Malondialdehyde （MDA）, Glutathione （GSH）, Superoxide Dismutase （SOD） and Glutathione Peroxidase （GSH-Px） were assayed by spectrophotometry in order to observe hepatoprotective effect of RLP. The results showed that, High-dosage RLP could significantly decreased liver index （P〈0.05） and spleen index （P〈0.01）. Both high-dosage RLP and middle-dosage RLP could significantly decreased ALP, AST and ALT in the serum （P〈0.01）, but that of in liver tissue was just the opposite. And the above 2 groups could significantly decreased MDA and increased GSH-Px, GSH and SOD content （P〈0.05 or P〈0.01） in liver tissue of CC14 poisoned mouse. RLP has hepatoproteetive effect on acute hepatic injury in mice induced by CCh.
出处
《中国农学通报》
CSCD
2012年第35期55-58,57-58,共4页
Chinese Agricultural Science Bulletin
基金
湖南省教育厅立项课题"金樱子多糖对急性药物肝损伤的保护作用研究"(湘教通[2010]244号-334)
关键词
金樱子多糖
四氯化碳
急性肝损伤
抗氧化
Rosa laevigata Michx Polysaccharide
Carbon Tetrachloride
acute hepatic injury
Antioxidant
