摘要
目的研究HBV转基因小鼠肝脏在四氯化碳（CCl4）急性损伤情况下,肝癌相关基因细胞色素P2E1（CYP2E1）表达水平的变化。方法选择8~10周龄HBV（-）[HBV（-）组]及HBV（＋）[HBV（＋）组]转基因小鼠各24只,按照1.0μL/g体质量腹腔注射CCl4（1∶4溶于橄榄油）建立急性肝损伤模型,另外选用正常健康小鼠8只作为对照组,仅给予生理盐水腹腔注射。各组分别于注射后3、6、12、24、48 h和72 h处死小鼠。采集各组大鼠肝组织样本,光镜下观察不同时间点肝脏组织学改变,并采用荧光定量PCR法测定各组大鼠肝组织中CYP2E1基因相对的m RNA水平,采用免疫组织化学和蛋白印迹技术观察各组大鼠肝脏组织中CYP2E1的表达水平。结果与对照组比较,HBV（-）组和HBV（＋）组,CCl4致急性肝损伤时,CYP2E1的m RNA及其蛋白表达水平明显升高,在注射CCl472 h达到峰值。与HBV（-）组比较,HBV（＋）组小鼠肝脏损伤程度较重,CYP2 E1基因和蛋白的表达水平均明显增高。结论 CCl4所致急性肝损伤HBV转基因小鼠CYP2E1基因的表达明显增加。该研究结果为HBV所致的肝损伤及肝癌的发病机制提供了依据。
Objective To study the CYP2 E1 gene expression in carbon tetrachloride（ CCl4）-induced acute liver injury in HBV transgenic mice. Methods Twenty four HBV（-） and twenty four HBV（ ＋） transgenic mice aged 8 to 10 weeks were selected for the present study. CCl4 intraperitoneal injection were performed to induce acute liver injury model. 8 normal clean-grad C57 BL /6 mice were taken as the control group. The control group received saline intraperitoneally. 3 h,6 h,12 h,24 h,48 h and 72 h after injection,respectively,the mice in each group were killed. The liver tissue samples of mice were collected. The liver histological changes at different time points in each group were observed under light microscope. The quantitative PCR methods were utilized to measure the relative m RNA levels of CYP2 E1 gene in liver tissue. Immunohistochemistry and Western blot techniques were used to observe tissue expression levels of CYP2 E1 in each group. Results Compared with the control group,both in HBV（-） group and in HBV（ ＋） group had severe liver damage,CYP2E1 gene and protein expression levels significantly increased. Conclusion In CCl4 induced acute liver injury of HBV transgenic mice,the CYP2E1 gene expression significantly increased. The results provided evidence for the HBV-induced liver damage and liver cancer pathogenesis.
出处
《新疆医科大学学报》
CAS
2016年第10期1272-1276,共5页
Journal of Xinjiang Medical University
基金
新疆医学动物模型研究重点实验室开发课题(XJDX1103-2013-07)
