摘要
目的:观察细脚拟青霉粗多糖(cPtPs)及其纯化多糖(PtPs)对四氯化碳(CCl4)诱导大鼠急性肝坏死的影响。方法:将Wistar大鼠分为4组(对照组、CCl4组、cPtPs+CCl4组和PtPs+CCl4组),分别用生理盐水、cPtPs和PtPs灌胃15 d,最后2 d,腹腔注射CCl4,16 h后全自动生化分析仪检测血清中丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)、总胆红素(TBIL)、直接胆红素(DBIL)和间接胆红素(IBIL);肝组织苏木素-伊红(HE)染色观察病变程度;黄嘌呤氧化酶法和硫代巴比妥酸法分别检测肝匀浆中超氧化物歧化酶(SOD)和丙二醛(MDA);甲基百里香酚蓝比色法测定肝细胞线粒体中Ca2+浓度;免疫组织化学法检测肝组织中α-平滑肌肌动蛋白(α-SMA)的表达。结果:与对照组比较,CCl4组血清中ALT、AST、TBIL、DBIL和IBIL的含量显著增加(P<0.05),HE染色肝组织变性坏死累及全小叶。与CCl4组比较,PtPs+CCl4组血清中ALT、AST、TBIL、DBIL和IBIL的含量显著下降(P<0.05);PtPs+CCl4组HE染色病变程度较轻,变性坏死局限于肝小叶的Ⅲ区;PtPs+CCl4组胞浆中SOD活性提高,MDA水平降低(P<0.05);PtPs+CCl4组和cPtPs+CCl4组线粒体中Ca2+浓度均下降(分别为P<0.05,P<0.01);PtPs+CCl4组α-SMA在肝组织的坏死区几乎无表达。结论:PtPs能显著减轻CCl4诱导的肝损伤,可能与PtPs抗脂质过氧化作用相关,效果优于cPtPs。
AIM： To investigate the protective effect of crude Paecilomyces tenuipes polysaccharides （cPtPs） and Paecilomyces tenuipes polysaccharides （PtPs） in a rat model of acute hepatic necrosis induced by carbon tetrachloride （ CCl4 ）. METHODS ： Wistar rats were divided into four groups （ control group, CCl4 group, cPtPs ＋ CCl4 group and PtPs ＋ CCl4 group）, the four groups were given intragastrically with normal saline, cPtPs and PtPs for 15 d, respectively. In the last two days, these groups except control group were injected peritoneally with CCl4 . Serum levels of alanine aminotransferase （ALT）, aspartate aminotransferase （AST）, total bilirubin （TBIL）, direct bilirubin （DBIL） and indirect.bilirubin （IBIL） were detected by automatic biochemistry analyzer. Pathological changes of hepatic tissue were assessed by hematoxylin - eosine （HE） staining. The levels of superoxide dismutase （SOD） and malondialdehyde （MDA） in liver homogenate were analyzed using xanthinoxidase and thio - barbituric acid, respectively. The concentration of Ca^2＋ in hepatocyte mitochondria was determined by colorimetric method. The expression of α- smooth muscle actin （α- SMA） was examined in hepatic tissue by immunohistochemical method. RESULTS： Compared with control group, serum levels of ALT, AST, TBIL, DBIL and IBIL in CCl4 group increased significantly, denaturation and necrosis implicated to the whole hepatic lobules. Compared with CCl4 group, serum levels of ALT, AST, TBIL, DBIL and IBIL in PtPs ＋ CCl4 group decreased significantly, denaturation and necrosis located in the third region of hepatic lobules, the level of SOD increased and MDA decreased （P 〈 0. 05 ） in endochylema. Concentration of Caz ~ in mitochondria decreased in PtPs ＋ CCl4 group and cPtPs ＋ CCl4 group （P 〈 0. 05, P 〈 0. 01, respectively）. Expression of α - SMA was found little in PtPs ＋ CCl4 group. CONCLU- SION ： PtPs, the effect is better than cPtPs, lessens CCl4 -
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2008年第9期1799-1803,共5页
Chinese Journal of Pathophysiology
基金
浙江省中医药科研基金资助项目(No.2004C121)
温州市科技计划资助项目(No.Y2004A008)
关键词
拟青霉
多糖类
大鼠
四氯化碳
肝损伤
Paecilomyces
Polysaccharides
Rats
Carbon tetrachloride
Liver injury
