摘要
用Box-Behnken中心组合试验设计和单因素试验对虫草多糖发酵工艺、水提醇沉工艺及脱蛋白工艺进行优化。结果表明:细脚虫草最佳发酵工艺条件为葡萄糖33.3g/L、蛋白胨29.2g/L、接种量3.9%。胞外多糖最佳提取工艺条件为浓缩,加3倍乙醇,pH为6.0,沉淀24h;胞内多糖最佳提取工艺条件为浸提比(m(菌丝)∶V水)1∶40(g/mL),提取时间90 min,浸提次数2次,浸提温度80℃。多糖纯化中Sevage法脱蛋白的最佳工艺为氯仿与正丁醇配比(V(氯仿)∶V(正丁醇))4∶1、Sevage试剂与发酵液配比(Vsevge试剂∶V发酵液)1∶2、振摇时间15min、脱蛋白次数3次。优化后胞外多糖产量提高了37.89%,胞内多糖产量提高了24.59%,纯化后蛋白脱除率在75%以上。
Using Box-Behnken central combination test design and single factor test were used to optimize the fermentation process of cordyceps polysaccharide,water extraction and alcohol precipitation process and deproteinization process.The results showed that the optimum fermentation conditions of Cordyceps tenuipes were glucose 33.3 g/L,peptone 29.2 g/L,inoculation amount3.9%.The optimum extraction conditions of Exopolysaccharides were concentration,3 times ethanol,pH 6.0 and precipitation for24 h.The optimum extraction conditions were 1∶40(g/mL),extraction time 90 min,extraction times 2 times,and extraction temperature 80℃.In the purification of polysaccharides,the optimum deproteinization techniques of Sevage were as follows:the volume ratio of chloroform to n-butanol was 4∶1,the volume ratio of Sevage reagent to fermentation broth was 1∶2,the shaking time was 15 min,and the deproteinization times was 3 times.After optimization,the yield of extracellular polysaccharide increased by 37.89%,that of intracellular polysaccharide increased by 24.59%,and the protein removal rate was more than 75%after purification.
作者
王小爱
梁建东
高小娇
蔡远俊
秦宇蒙
田维毅
WANG Xiao-ai;LIANG Jian-dong;GAO Xiao-jiao;CAI Yuan-jun;QIN Yu-meng;TIAN Wei-yi(College of Basic Medicine,Guizhou University of Traditional Chinese Medicine,Guizhou,Guiyang 550025,China;Center for Mycomedicine Research,Guizhou University of Traditional Chinese Medicine,Guizhou,Guiyang 550025,China)
出处
《食品与机械》
北大核心
2020年第11期158-164,216,共8页
Food and Machinery
基金
贵州省千层次人才项目(编号:2014)
贵州中医药大学校级科研项目(编号:贵中医科院内[2014]28号)
贵州省科技基金项目(编号:黔科合基础[2018]1014)
贵州省普通高等学校工程研究中心建设项目(编号:黔教合KY字[2015]337)
贵州省科技创新人才团队项目(编号:黔科合平台人才[2020]5010)
贵州省大学生创新创业训练计划项目(编号:贵中医大创合字[2015]34号)
关键词
虫草
多糖
液体发酵
水提醇沉
Sevage法
Cordyceps
polysaccharides
liquid fermentation
supernatants of alcohol extracting-water precipitation
sevage method
