摘要
使用液态发酵培养基直接诱导富集活性成分可以高效获得食用菌活性物质。本研究设计一种胞外液态灵芝酸A诱导富集培养基,诱导富集三萜类化合物灵芝酸A。通过单因素试验,研究了培养基中的碳源、氮源、生长因子对灵芝酸A产量的影响,确定了最佳诱导富集培养基组分及其浓度。结果表明,灵芝酸A诱导富集培养基配方为:葡萄糖25~30 g/L,组氨酸0.5~0.7 g/L,复合维生素B 10~50 mg/L。
The active components of edible mushroom could be obtained efficiently by direct induction using the liquid fermentation culture medium.In this research,a liquid culture medium inducing the enrichment of ganoderma acid A was designed to induce the enrichment of triterpenoid of ganoderma acid A.The single factor experiments were employed to research the effects of carbon source,nitrogen source and growth factor in culture medium on the production of ganoderma acid A,therefore the components and contents of culture medium were determined.The formula of medium inducing the enrichment of ganoderma acid A was determined as follows:glucose of 25~30 g/L,histidine of 0.5~0.7 g/L,and complex vitamins B of 10~50 mg/L.
作者
丁舒
崔瀚元
陈颖
张越
张志军
陈晓明
DING Shu;CUI Han-yuan;CHEN Ying;ZHANG Yue;ZHANG Zhi-jun;CHEN Xiao-ming(Institute of Agricaltural Products Preservation and Processing Science and Technology,Tianjin Academy of Agricultural Sciences,Tianjin 300384,China)
出处
《保鲜与加工》
CAS
2021年第8期50-54,共5页
Storage and Process
基金
青年科研人员创新研究重点项目(2020003)
天津市林业果树研究所所长基金项目(SZJJ2016002)。
关键词
液态发酵
培养基
诱导富集
灵芝酸A
liquid fermentation
culture medium
inducing enrichment
ganoderma acid A
