摘要
采用考马斯亮蓝G-250染色法对核桃粗蛋白中可溶性蛋白质含量进行了测定,结果表明,蛋白质浓度为10mg/mL^80mg/mL时与吸光度值有良好的线性关系,考马斯亮蓝G-250染色法简单、快速、灵敏,且相对较为准确,是测定低浓度蛋白质含量的有效方法。通过此方法,测得的核桃水溶性蛋白平均含量为8.37%。
The soluble protein content in walnut was determined by using coomassie brilliant blue G-250.Result showed there was a good linear relationship between protein concentration and absorbance value within the concentration value of 10mg/mL～80mg/mL.The Bradford method was simple,rapid,sensitive and relatively accurate,which was an effective method for measuring low concentrations protein content.By this method,the measured average protein content of walnut was 8.37％.
出处
《中国酿造》
CAS
2013年第12期131-133,共3页
China Brewing
基金
贵州省农业攻关项目(黔科合NZ[2013]3016号)
关键词
核桃
蛋白质
考马斯亮蓝
walnut
protein
coomassie brilliant blue （CBB）
