摘要
目的:揭示荷瘤小鼠气虚证甲状腺基因表达的特征。方法:采用荷瘤小鼠及其标准化四诊及辨证方法,应用GeneChip Mouse Exon1.0STArray等技术,检测H22荷瘤小鼠早期邪毒壅盛证和气虚证、中期阳气虚证、中晚期气阴阳虚证共3阶段4个证候甲状腺基因表达的差异,重点关注早期气虚证上下调且表达量高的基因。结果:气虚证荷瘤小鼠甲状腺独特上调基因有33个,其中表达较高的基因6个,分别为Mta2d1、Comd3、Ucp1、Psmc6、LOC665964、Cyp2j11;独特下调的基因有31个,其中正常组表达较高的基因有5个,分别为Lcn2、Arntl、Mansc1、Hk1、Rpl29。结论:肿瘤发生后,H22荷瘤小鼠甲状腺存在大量的基因表达改变,其中早期气虚证独特上调或下调基因,可能与气虚证本质有关。
Objective： To reveal the character of gene expression in thyroid of H22 tumor-bearing mice belonging to qi-deficiency syndrome. Methods： By the quantitative four diagnosis and syndrome differentiation methods and Gene Chip Mouse Exon 1.0 ST Array, we observed thyroid gene expression in H22 tumor-bearing mice about poisonous pathogenic factors syndrome and qi-deficiency syndrome in earlier stage, yang-asthenia syndrome in intermediate stage, and syndrome of asthenia of qi, yin and yang in advanced stage. The differences of thyroid gene expression of the four syndromes in the three stages were analyzed, and up-or-down-regulating gene＇ s high expression on qi-deficiency syndrome in earlier stage was especially paid close attention. Results ： Thirty-three up-regula- ting genes in qi-deficiency syndrome were obtained, and six of them were highly expressed, which were Mta2d1, Cored3, Ucpl, Psmc6, LOC665964 and Cyp2j11. Thirty-one down-regulating genes were obtained, and five of them were highly expressed in normal control group, which were Lcn2, Arntl, Manscl, Hk1 and Rp129. Conclusion ： After the tumorigenesis, the changes of gene expression are distinguished in thyroid of H22 tumor - bearing mice. Those up-or-down-regulating expressed genes in earlier stage could be related to the essence of qi-deficiency syndrome.
出处
《山西中医》
2008年第8期44-47,共4页
Shanxi Journal of Traditional Chinese Medicine
基金
上海市科委国际合作项目(项目批准号:064309052)
2006上海市教委学科建设项目
关键词
小鼠
肿瘤
甲状腺
证候
气虚
基因
实验研究
mice, turnout, thyroid, symptoms and signs, qi- deficiency syndrome, gene, Gene Chip
