摘要
目的:提取漏芦多糖,分析其单糖组分并测定其质量分数,为以后漏芦多糖研究提供参考。方法:水提醇沉法提取粗多糖,Sevag法除蛋白,高效阴离子交换色谱-脉冲安培检测(HPAEC-PAD)法测定其单糖组成,苯酚-硫酸法测定多糖质量分数。结果:漏芦多糖的单糖组成为葡萄糖、阿拉伯糖和果糖,其摩尔比为1∶1.61∶2.21。以单糖混合物为对照品测得漏芦多糖中总糖的质量分数为95.78%。结论:HPAEC-PAD法具有样品不用衍生、灵敏度高、精密度好等优点,适合多糖类样品中单糖的分析;苯酚-硫酸法实验操作简便,准确可靠,可用于多糖含量测定。
Objective： To analyze the monosaccharide composition in the polysaccharides from Rhaponticum uniforum, determine the content of monosaccharide,and provide some references for further research. Method： The monosaccharide composition was determined by high performance anion exchange chromatography with pulsed amperometric detection （HPAEC-PAD）. Phenol-sulfuric acid method was used for the determination of the content of polysaccharide. Result： The monosaccharides composition in polysaccharides from R. uniforum are glucose, arabonose and fructose. Their molar ratios are 1 ： 1.61 ： 2.21. The content of polysaccharide is 95. 78% ,taking the mixture of monosaccharide compositions as reference substances. Conclusion： HPAEC-PAD can be used to analyze the monosaccharide composition in the polysaccharide with high precision, and the method of phenol-sulfuric acid is simple, convenient and reliable.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2008年第11期1284-1286,共3页
China Journal of Chinese Materia Medica
基金
辽宁省教育厅科研基金项目(02200953)
关键词
漏芦
多糖
高效阴离子交换色谱
脉冲安培检测
苯酚-硫酸法
Rhaponticum uniforum
polysaccharide
high performance anion exchange chromatography
pulsed amperometric detection
phenol-sulfuric acid method
