摘要
目的采用高效阴离子交换色谱-脉冲安培电化学检测法（HPAEC-PAD）对巴戟天不同生长期中的6种寡糖（葡萄糖、果糖、蔗糖、1-蔗果三糖、耐斯糖、1F-果呋喃糖基耐斯糖）进行检测,对其动态积累规律进行分析,为研究巴戟天的最佳采收期提供参考。方法利用高效阴离子交换色谱-脉冲安培电化学检测法,色谱条件如下：Hamilton RCX-10色谱柱（4.1 mm×250mm,7μm）,100 mmol·L-1Na OH溶液（A）-100 mmol·L-1Na OH溶液,500 mmol·L-1Na OAc混合溶液（B）为流动相梯度洗脱,流速0.8 m L·min-1,柱温35℃,进样量25μL。结果随着生长年限的增加,巴戟天根中的单糖呈现减少的趋势,蔗糖则逐渐增加,聚合度为3或以上的1-蔗果三糖、耐斯糖、1F-果呋喃糖基耐斯糖则在1~5年间渐增加,然后缓慢降低。结论不同生长年限巴戟天中6种寡糖成分的组成及含量相差较大,提示该类成分的合成与生长年限有一定的关系,为制定适宜的采收期提供参考。
OBJECTIVE To determine the contents of six oligosaccharides, i.e. glucose, fructose, sucrose, 1-kestose, nystose, 1 r-fructofuranosylnystose, in the roots of Morinda officinalis in different growing periods by HPAEC-PAD method. METHODS The separation was performed on Hamilton RCX-10 column（4. 1 mm × 250 mm, 7 μm） with gradient elution using mobile phase composed of 100 mmol · L-1 NaOH（A） and mixture of 100 mmol· L-1 NaOH and 500 mmol · L-1 NaOAc （B） at a flow rate of 0. 8 mL ·min-1 and the temperature was kept at 35 23. The injection volumn was 25 μL. The gradient elution program was as follows ： 0 - 5 min,1%B;5-15 min,1% -9% B;15 -20 min,9% -12% B;20-25 min,12% -16% B;25 -40 min,16% -50% B;40-43 min,50% B ;43 -46 min,50% - 1% B. RESULTS With the increase of growing periods, the content of monosaccharide progres- sively reduced while that of sucrose gradually increased. The contents of oligosaccharides with polymerization degree of 3 or greater, in- cluding 1-kestose, nystose, and 1F-fructofuranosylnystose, slowly increased during 1 -5 years, then slowed down. CONCLUSION The contents of six oligosaccbarides in the roots of Morinda officinalis vary greatly in different growing periods, indicating that the bio- synthesis of oligosacchride in Morinda officinalis is related to growing periods. The result provides a reference for determination of ap- propriate collection time.
出处
《中国药学杂志》
CAS
CSCD
北大核心
2015年第21期1912-1916,共5页
Chinese Pharmaceutical Journal
基金
广东省自然科学基金重点资助项目(0610506)
