摘要
目的:检测纤维蛋白胶复合β-磷酸三钙/磷酸二氢钙复合人工骨材料的物理学性能,评价纤维蛋白胶对β-磷酸三钙/磷酸二氢钙骨水泥性能的影响,以及其作为注射型复合人工骨用于修复骨缺损的可行性。方法:实验于2006-12/2007-06在南方医科大学珠江医院中心实验室和华南理工大学生物材料实验室完成。①材料:β-磷酸三钙由上海瑞邦生物材料有限公司提供,磷酸二氢钙为东泰化工赠,纤维蛋白胶购自广州倍绣生物技术有限公司。②复合材料制备:将β-磷酸三钙/磷酸二氢钙骨水泥按3∶1的比例充分混合后,与纤维蛋白胶按凝固后的体积2∶1体积比混合,制成复合人工骨材料。③观察指标:测定复合材料的凝固时间,抗压强度,抗稀散性能,并用扫描电镜观察其煅烧前后的显微结构特征,以未加纤维蛋白胶的磷酸钙水泥为对照(CPC组)。结果:复合人工骨材料的平均初凝时间长于CPC组(P<0.004),终凝时间在初凝时间后2～4 min;复合材料的抗压强度为(14.72±1.81)MPa。复合材料较CPC组有良好的抗稀散性能,扫描电镜发现,纤维蛋白胶贯穿于磷酸钙水泥晶体间,并将磷酸钙水泥晶体紧密连接。煅烧后复合材料的孔径有增大,空隙率为57.28%,并且微孔之间有空隙互相贯通。结论:该骨水泥复合材料凝固时间符合临床操作的需要;抗压强度达到松质骨强度的要求;煅烧后磷酸钙水泥的空隙率明显提高,有利于材料的降解。
AIM： To study the physics performance of fibrin glue compound β-tricalcium phosphate （β-TCP）/monocalcium phosphate artificial bone material, evaluate the effect of fibrin glue on β-TCP/monocalcium phosphate, and investigate the feasibility of applying it as an injectable artificial bone to repair bone defects. METHODS： From December 2006 to June 2007, the experiment was carried out in the Central Laboratory, Zhujiang Hospital of Southern Medical University and the Biomaterials Laboratory, South China University of Technology.（1）β-TCP was offered by Shanghai Rebone Biomaterials CO., LTD. monocalcium phosphate was offered by Dongtai Chemical Industry Co., Ltd. Fibrin glue was purchased from Guangzhou Bioseal Biotech Co.,Ltd.（2）β-TCP/monocalcium phosphate was full mixed by 3：1, then mixed with fibrin glue at the ratio of 2：1 to prepare the compound artificial bone. （3）The coagulation time, compressive strength, resistance scattered performance of composite material were determined, and the characteristic of its microstructure was observed before and after calcinations by using scanning electron microscope. While those calcium phosphate cement without the fibrin glue was served as control group.RESULTS： The average initial setting time of the compound materials was longer than that of control group （P 〈 0.004）, and the final setting time was 2-4 minutes after the initial setting time; the compressive strength of composite materials was （14.72±1.81） MPa. Compared with the control group, composite materials had good performance against scattered, and scanning electron microscope result revealed that the fibrin glue penetrated throughout calcium phosphate crystals, which were connected tightly. After calcination, the porosity of compound materials increased to 57.28%, and the gap between the micropores was mutual linked.CONCLUSION： The bone cement composite material is suitable for the clinical operation in aspects of coagulation time; the compressive strength fits for the can
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2007年第48期9633-9636,共4页
Journal of Clinical Rehabilitative Tissue Engineering Research
基金
南方医科大学院长基金资助课题(2006)~~
