摘要
目的掌握组织工程骨的构建,观察兔成骨细胞在聚乳酸与骨髓基质干细胞复合后形成的人工骨材料体外培养的生物学行为反应。方法将兔成骨细胞与聚乳酸人工骨进行体外复合培养,采用倒置相差显微镜、扫描电镜进行检测,观察兔成骨细胞在聚乳酸人工骨上的黏附能力和生物学行为。结果将兔成骨细胞和聚乳酸人工骨体外复合培养6~18周间,成骨细胞贴附于人工骨表面,可观察到细胞间形成一定的连接。结论成骨细胞可以在复合人工骨材料中黏附、增殖和分化,成骨细胞复合人工骨材料可以在体内成骨。
Objective To discuss the effects on osteoblast biological behavior in vitro circumstance of the artificial bone materials which is composited with Marrow stromal stem cells and polylactic acid.Methods Polylactic acid artificial bone and rabbit osteoblasts were co-cultured in vitro.Inverted phase contrast microscope,scanning electron microscope,transmission electron microscope and fluorescence microscope were used to observe the adhesive capacity and biological behavior of rabbit osteoblast on Nacre-polylactic acid artificial bone.Results By means of pseudopods,osteoblast attached to the surface of polylactic acid artificial bone 5 days after co-cultured.Transmission electron microscope showed that osteoblast eumorphism,their were abundant rough surfaced endoplasmic reticulum and mitochondria in cytoplasm,and,their were Connections between cells.Conclusion Composite artifical bone had the strongest effect on the attachment,proliferation and differentiation of osteoblast and could promote osteogenic potential in vitro.
出处
《内蒙古医学杂志》
2015年第10期1164-1166,1148,共3页
Inner Mongolia Medical Journal
基金
国家自然科学基金应急管理项目(编号:81450037)
内蒙古医科大学院内基金(2013YJJ09)
关键词
组织工程
聚乳酸
成骨细胞
tissue engineering
polylactic acid
osteoblast
