摘要
目的 比较1-乙基-3-（3-二甲氨基丙基）碳化二亚胺[1-ethyl-3-（3-dinethylami-nopropyl） carbodimide，EDC]/N-羟基琥珀酸亚胺（N-hydroxysuccininide，NHS）和京尼平对脱细胞牛心包膜的交联效果，为选择最佳交联剂提供依据。 方法 取市售健康成年黄牛心包膜，采用高/低渗溶液法脱细胞，HE染色观察明确无细胞残留。将脱细胞牛心包膜分别采用EDC/NHS（EDC/NHS组）和京尼平（京尼平组）进行交联处理后，行扫描电镜、厚度、交联度、力学性能、变性温度测定和体外降解与细胞毒性实验，评价不同交联剂对脱细胞牛心包膜的改性效果。以单纯脱细胞牛心包膜（脱细胞组）、正常牛心包膜（空白对照组）作为对照。 结果 扫描电镜观察示，两交联组胶原纤维呈相对规则的网状结构，孔径为10-20 μm。与脱细胞组及空白对照组相比，两交联组牛心包膜厚度、变性温度均显著增加（P〈0.05），但两组间比较差异无统计学意义（P〉0.05）。两交联组交联度比较差异亦无统计学意义（t=0.205，P=0.218）。各时间点两交联组降解率均显著低于空白对照组及脱细胞组（P〈0.05）。两交联组弹性模量和断裂应力均较脱细胞组显著下降（P〈0.05），且接近空白对照组（P〉0.05），而断裂伸长率均较空白对照组和脱细胞组显著增加（P〈0.05）；以上指标EDC/NHS组和京尼平组间比较差异均无统计学意义（P〉0.05）。细胞培养3、5 d时京尼平组细胞毒性均为1级，而EDC/NHS组5 d时达2级。 结论 京尼平交联脱细胞牛心包膜后生物性能优于EDC/NHS。
Objective To investigate the effects of modification of acellular bovine pericardium with 1-ethyl-3-（3-dinethylami-nopropyl） carbodimide （EDC）/N-hydroxysuccininide （NHS） or genipin and find out the best crosslinking reagent. Methods The cellular components of the bovine pericardiums were removed. The effects of decellularization were tested by HE staining. The acellular bovine pericardiums were crosslinked with EDC/NHS （EDC/NHS group） or genipin （genipin group）. The properties of the crosslinked acellular matrix were evaluated by scanning electron microscope （SEM）, matrix thickness, crosslinking index, mechanical property, denaturation temperature, enzymatic degradation, and cytotoxicity test before and after the crosslinking. Acellular bovine pericardium （ABP group） or normal bovine pericardium （control group） were harvested as controls. Results SEM showed that collagen fibers were reticulated in bovine pericardial tissues after crosslinked by EDC/NHS or genipin, and relative aperture of the collagen fiber was from 10 to 20 μm. The thickness and denaturation temperature of the scaffolds were increased significantly after crosslinking with EDC/NHS or genipin （P〈0.05）, while there was no significant difference between EDC/NHS group and genipin group （P〉0.05）. The difference had no statistical significance in crosslinking index between EDC/NHS group and genipin group （t=0.205, P=0.218）. The degradation rate in EDC/NHS group and genipin group was significantly lower than that in ABP group and control group （P〈0.05）. Elastic modulus and fracture stress in EDC/NHS group and genipin group were significantly lower than those in ABP group （P〈0.05）, but there was no significant difference among EDC/NHS group, genipin group, and control group （P〉0.05）. The break elongation in EDC/NHS group and genipin group were significantly increased than those in ABP group and control group （P〈0.05）. The difference had no statistical significance in stability and m
出处
《中国修复重建外科杂志》
CAS
CSCD
北大核心
2015年第10期1301-1306,共6页
Chinese Journal of Reparative and Reconstructive Surgery
基金
郑州市科技攻关项目(121PPTGG498-2)~~
