摘要
目的在碳化二亚胺交联的去细胞牛心包膜上采用3种方式进行肝素改性，即共价键合肝素、离子结合肝素以及共价-离子联合负载肝素，比较这3种肝素化方式对牛心包膜抗凝血性能的影响，并筛选出最佳肝素化方式。方法通过溶血试验、血小板黏附实验、体外凝血测试以及复钙时间测定观察3种方式肝素化的基质抗凝血性能和血栓形成情况，以评价其血液相容性。结果综合上述4项检测的结果，共价-离子联合负载肝素改性的抗凝血性能优于共价键合肝素、离子结合肝素这2种单独负载肝素方式，溶血率〈5％，电镜照片无血小板黏附，体外生理盐水浸泡15d后，仍保持良好的抗凝血活性。结论共价-离子联合负载肝素，将离子结合肝素活性好、共价键合肝素稳定性强的优点结合起来，取长补短，使这种方式肝素改性的牛心包具有良好的血液相容性。
Objective To investigate the anticoagulant properties of heparinized bovine pericardiums and make the best choice among three ways of heparinization： Heparinized EDC treated bovine peficardiums; Heparinized PEI treated bovine pericardiums; Heparinized EDC-PEI treated bovine pericardiums. Methods The blood compatibility of heparinized bovine pericardiums was assessed in vitro by hemolysis test, platelet attachment test, PT/APTT and recalcifieation time. Results Heparinized EDC-PEI treated bovine peficardiums were the best in terms of the anticoagulant properties, which had low hemolysis ratio （〈5%） and no platelet attachment, even that PT/APTT and recaleification time were prolonged obviously for 15 days. Conclusion Heparinized EDC-PEI treated bovine pericardiums have stable and promising blood compatibility.
出处
《国际生物医学工程杂志》
CAS
北大核心
2010年第3期152-156,175,共6页
International Journal of Biomedical Engineering
关键词
牛心包
肝素化
碳化二亚胺
聚乙烯亚胺
抗凝血性能
Bovine pericardiums
Heparinization
EDC
Polyethylenimine
Anticoagulant property
