摘要
目的探讨白细胞介素1相关基因(IL-1A)-889/NcoⅠ基因多态性和吸烟因素与汉族人慢性牙周炎(CP)易感性的关系。方法收集口腔科门诊山东籍汉族慢性牙周炎患者142例和95名对照者,通过问卷调查获得吸烟的资料。采集颊粘膜拭子提取DNA,采用序列特异引物聚合酶链反应和聚合酶链反应-限制性片段长度多态性方法,进行IL-1A-889/NcoⅠ位点基因型测定,分析各基因型分布;将基因型与吸烟因素进行交互作用分析。结果142例患者中重度慢性牙周炎46例,中度45例,轻度51例。IL-1A-889/NcoⅠ的等位基因2在重度慢性牙周炎中的检出率明显高于对照组,差异有统计学意义(P<0.01)。而中度慢性牙周炎、轻度慢性牙周炎与对照组之间的分布差异无统计学意义。吸烟能明显提高牙周病的发生,含有等位基因2的基因型与吸烟有交互作用(OR=10.413,P=0.000;OR=5.315,P=0.004)。结论IL-1A-889/NcoⅠ等位基因2可能与汉族重度慢性牙周炎遗传易感性相关,吸烟因素与牙周病发生有关,含有等位基因2的吸烟个体更易患牙周炎。
Objective To investigate the association of interleukin - 1A - 889 gene polymorphisms and smoking with the susceptibility to chronic periodontitis in Han nationality. Methods 142 chronic periodontitis （ CP ） patients and 95 healthy controls were selected and the information of whether or not smoking was obtained through questionnaire survey; DNA was extracted from these buccal swabs and genotypes of the IL - 1A - 889/Nco Ⅰ were determined by sequence specific primers polymerase chain reaction （SSP - PCR ） and PCR restriction fragment length polymorphism （PCR - RFLP）. Then the interaction between the genotypes of L - 1A - 889/Nco Ⅰ and smoking was analysed. Results There were 46 severe chronic periodontitis （CP） patients, 45 moderate CP patients and 51 mild CP patients in 142 chronic periodontitis （CP） patients. Frequencies of allele 2 for IL- 1A - 889/Nco Ⅰ were higher in severe CP patients than in healthy controls, and the difference was statistically significant（P 〈 0.01）. But there were no such significant differences either between moderate CP patients and healthy controls or between mild CP patients and healthy controls. Smoking can significantly increase the incidence of periodontal disease, and there were interaction between the genotypes containing allele 2 and smoking（ OR = 10. 413, P = 0. 000; OR = 5. 315, P = 0. 004）. Conclusion IL- 1A- 889 may be a risk indicator for the susceptibility to severe chronic periodontitis in Han nationality, and smoking was associated with CP and may represent a risk factor for this disease. Individuals carrying the positive genotype have significant risk for CP.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2007年第9期1092-1094,共3页
Chinese Journal of Public Health
基金
世界卫生组织与加拿大国际合作资助项目
关键词
慢性牙周炎
汉族
白细胞介素1
基因多态性
吸烟
chronic periodontitis
Han nationality
interleukin - 1
genetic polymorphism
smoking
