摘要
目的:研究对比了用不同方法提取红松、樟子松和红皮云杉等3种松科植物叶片基因组DNA的效果,以寻找适合提取松科植物叶片基因组DNA的方法。方法:分别比较了用传统的CTAB法、SDS法以及3种改良的CTAB法提取的上述3种松科植物叶片基因组DNA的电泳、纯度和酶切效果。结果:采用不同方法提取的3种松科植物叶片基因组DNA的质量差异较大。结论:常规CTAB法和SDS法无法提取出高质量松科植物基因组DNA,而改良后的CTAB法的提取效果较好。
Objective： In order to find the suit method of genome DNA extraction from Pinaceae, the different methods of genome DNA extraction from there kind of Pinaceae plants （Pinus koraiensis, Pinus sylvestris var. mongolica, Picea koraiensis） were studied. Methods： The result of electrophoresis, purity and enzyme digesting of genome DNA of three kind of Pinaceae were compared. Results： The quality of DNA by different methods of genome DNA extraction was different. Conclusion： The results showed that the improving CTAB was better than conventional CTAB and SDS.
出处
《生物技术通讯》
CAS
2007年第2期263-266,共4页
Letters in Biotechnology
基金
国家高技术研究发展计划项目(2002AA241080)
东北林业大学校基金课题
