摘要
提取基因组DNA是开展芍药分子生物学研究中一项最为基础的工作。本研究通过常规CTAB法和近几年应用较为普遍的试剂盒法,对芍药基因组DNA进行了提取,并对2种方法所提取的DNA进行了浓度、程度、琼脂糖凝胶电泳和PCR扩增比较。结果表明,常规CTAB法提取的DNA浓度较高,为128.08-253.63 ng/ul,但纯度较低,而试剂盒法提取的DNA虽平均浓度较低,为3.51ng/ul,但纯度较高;电泳和PCR结果均表明,试剂盒法提取的DNA能扩增出目的片段,是较好的一种提取方法。
Extracting genomic DNA from Paeonia lactiflora is one of the most basic work in the research of molecular biology. Thisstudy through the conventional CTAB method and Reagent kit,genomic DNA were extracted and concentration of DNA,agarose gel elec-trophoresis and PCR amplification were compared.. The results showed that DNA concentration by CTAB method was higher,128.08-253.63 ng/ul and DNA concentration by reagent kit was lower,3.51ng/ul,but electrophoresis and PCR amplification resultsshowed that DNA extraction kit can amplified fragment, is a better extraction method.
出处
《现代园艺》
2016年第18期5-6,共2页
contemporary horticulture
