摘要
黄连木叶片中酚类化合物、色素、多糖和其它次生代谢物质含量较高,这给黄连木基因组DNA提取带来困难。为了从黄连木叶片中提取高质量基因组DNA,本文运用CTAB法和改良CTAB法提取黄连木基因组DNA,通过定性、定量及PCR分析检测所提取DNA的质量。结果表明:改良CTAB法提取的DNA电泳条带清晰无RNA等污染,OD260和OD280比值在1.8左右,RAPD扩增条带清晰、无弥散现象、亮度均一。说明改良CTAB法提取的DNA纯度较高,适用于PCR扩增反应。
It is difficult to extract genomic DNA due to the existence of phenolic compounds,pigments,polysaccharides,and other secondary metabolites in the leaves of Pistacia chinesis Bunge.In order to obtain its high-quality genomic DNA,CTAB method and improved CTAB method were used to extract its genomic DAN,and then the quality of extracted DNA were detected by qualitative analysis,quantitative analysis and PCR analysis.The tesults showed that the extracted DNA by improved CTAB method were clear and had no RNA contamination by the electrophoresis.The ratio of OD260 to OD280 was around 1.8.RAPD bands amplified were clear and had non-diffusin phenomena.The DNA isolated by improved CTAB method is suitable for PCR amplification.
出处
《河南科技大学学报（自然科学版）》
CAS
北大核心
2010年第3期71-73,共3页
Journal of Henan University of Science And Technology:Natural Science
基金
国家自然科学青年基金项目(30700078)
河南科技大学博士启动基金项目(09001062)
河南科技大学SRTP资助项目(2009130)
