摘要
目的:采用SRAP(sequence related amplified polymorphism)分子标记技术对不同产地蒙古黄芪进行遗传关系研究,为蒙古黄芪的种质资源评价提供一定的依据。方法:对来源于12个产地的蒙古黄芪的样品进行了SRAP多态性研究。结果:从80个引物组合中筛选出扩增产物条带信号强,重现性好,特征性好的16个引物组合,扩增得到141条条带,聚类分析结果显示,12份材料可以分为2个大类。结论:蒙古黄芪居群间遗传分化明显,SRAP技术可以有效用于蒙古黄芪的亲缘关系分析。
Objective:To study the genetic relationship of Astragalus membranaceus var.mongholicus in different producing area and provide theoretical basis for the evaluation of Astragalus germplasm resources.Method:Through quence-related amplified polymorphism(SRAP) analysis,the systematic diagram of genetic relationship was constructed by UPGMA method.Result:A total of 141 SRAP markers were scored.By the use of UPGMA cluster analysis with genetic distance,Astragalus could be divided into two provenance plots of Gans...
出处
《中国中药杂志》
CAS
CSCD
北大核心
2009年第4期382-385,共4页
China Journal of Chinese Materia Medica
基金
国家"十一五"科技支撑计划项目(2006BAI08B05-03)
关键词
蒙古黄芪
SRAP标记
遗传关系
Astragalus membranaceus var mongholicus
SRAP
genetic relationship
