摘要
用CTAB法从感染草莓镶脉病毒(SVBV)的草莓叶片中提取总DNA,设计3对特异性引物扩增SVBVCP基因的3个片段,分别克隆并测序。经序列拼接得到SVBVCP基因完整序列,全长1407nts,编码468个aa。将它与美国报道的SVBV(NC001725)以及花椰菜花叶病毒属其它成员的CP基因相比较,结果表明,SVBVCP基因与SVBV(NC001725)CP基因序列相似性最高,达83.4%;而与花椰菜花叶病毒属其它成员的CP基因序列相似性均较低,仅为27.1%～33.2%。构建SVBV及其同属其它成员CP基因的系统关系树,结果显示中国SVBV与SVBV(NC001725)单独形成一个分支,而与其同属其它成员的亲缘关系均较远。
The total DNA was extracted from strawberry leaves infected with SVBV by CTAB method. Three primer pairs were designed to amplify 3 fragments of SVBV CP gene respectively, and then they were cloned and sequenced. The complete sequence of SVBV CP gene was acquired by splicing the 3 partial sequences. The full length of SVBV CP gene was 1 407 nts, encoding 468 amino acids. Comparing SVBV CP gene with SVBV （NC001725）reported in America and the other members of Caulimovirus, the result showed that SVBV CP gene shared the highest sequence similarity （83.4%） with SVBV （NC001725）, while had relatively lower nucleotide sequence similarity （27. 1% -33.2% ） to the other members of Caulimovirus. A phylogenetic tree based on alignment of CP nucleotide sequences of SVBV and the other members of Caulimovirus was constructed. The result indicated that the SVBV from China and SVBV （NC001725） reported in America clustered into a separate branch, and they had relatively distant relationship with the other members of Caulimovirus.
出处
《安徽农业大学学报》
CAS
CSCD
北大核心
2009年第2期315-318,共4页
Journal of Anhui Agricultural University
基金
国家自然科学基金(30740033)
安徽省教育厅自然基金重点项目(KJ2008A133)共同资助
关键词
草莓镶脉病毒
CP基因
克隆
序列分析
strawberry vein banding virus
CP gene
clone
sequence analysis
