摘要
目的探讨三七皂苷Rg1对大鼠局灶性脑缺血再灌注损伤后大脑皮质中BDNFmRNA表达的影响。方法♂SD大鼠36只随机分为脑缺血再灌注模型组、三七皂苷Rg1组和尼莫地平组,采用线栓法制作模型,各组分别于术后1、3、5 d随机取4只大鼠处死后,取出脑组织,经石蜡包埋切片,片厚5μm。用地高辛标记的寡聚核苷酸探针进行原位杂交实验,用HPIAS-100高清晰度彩色病理图文报告分析系统测量分析大鼠大脑皮质的BDNFmRNA的含量和阳性神经元数目的变化。结果在脑缺血再灌注后不同的时间段上,与模型组比较,三七皂苷Rg1均能增加大脑皮质的BDNFmRNA含量及其阳性神经元的数量。用药3 d时,三七皂苷Rg1的作用达到高峰,且其作用在各时间段上均强于尼莫地平;5 d后,虽然BDNFmRNA的含量及阳性神经元的数量有一定程度的下降,但仍与三七皂苷Rg1组1 d时的表达水平相当,且三七皂苷Rg1组5 d时的表达水平仍强于模型组1 d和3 d时表达的水平及尼莫地平组1 d时的水平。结论三七皂苷Rg1通过上调大鼠脑缺血再灌注损伤后,大脑皮质BDNFmRNA的含量和阳性神经元的数量,可发挥其治疗作用,疗效强于阳性对照药尼莫地平。
OBJECTIVE To study the effect of notoginsenoside - Rg1 （NRg1） on expression of brain - derived neurotrophic factor messenger ribonucleic acid （BDNFmRNA） in cerebrum cortex after middle cerebral artery occlusion reperfusion （MCAO/R） injury in rat. METHODS 36 SD male rats,weighing 280 -320 g,were divided into MCAO/R model group,NRg1 therapy group and nimodipine therapy group randomly. The MCAO/R model rats were made by thread - occluded method. Four rats were randomly taken from each groups and sacrificed with brain broken out as specimen at 1,3,5 days after treated with medicine. The brain tissues were embedded by olefin and sliced into section of 5 μm thickness. The sections were stained by oligoriboprobes which were signed by digoxingenin in 3＇ side, all sections were under the same condition according to the procedure of hybridisation method in situ. The BDNFmRNA content and positive neurons in cerebrum cortex in each rat were observed and counted by HPIAS - 100 analysis system. RESULTS Compare with the model group, NRg1 treated groups can prominently increase BDNFmRNA content and BDNFmRNA positive neurons amount that in the cerebrum cortex at different period after rat MCAO/R injury. The efficacy of NRgl peak after 3 days. As well as the function of NRgl on increasing both BDNFmRNA content and positive neurons amount were superior to that of the positive control medi- cine （nimodipine） in each different treated period. Compare with the 3 days treated period, although it had declined in some extent that expression of BDNFmRNA content and positive neurons amount in 5 days NRg1 treated period, their expression levels was still better than that in 1 day or 3 days model group and the 1 day positive control group,which was similar to that of 1 day NRg, treated period and 3 days nimodipine treated period. CONCLUSION NRgl can up -regulate the expression of BDNFmRNA content and positive neurons amount in the cerebrum cortex after MCAO/R injury, which can give rise to protective effects for ischemic b
出处
《华西药学杂志》
CAS
CSCD
北大核心
2007年第2期160-162,共3页
West China Journal of Pharmaceutical Sciences
基金
云南省自然科学基金项目(No.2002C0051)
