摘要
目的 研究外源性神经生长因子 (NGF)对大鼠脑缺血再灌注后生长相关蛋白 43 (GAP 43 )和突触素 p3 8表达的影响。 方法 成年健康雌性Wistar大鼠 5 4只 ,随机分为NGF治疗组、对照组和假手术组。采用线栓法建立大脑中动脉缺血再灌注动物模型 ,应用免疫组织化学方法观察NGF对脑缺血再灌注后GAP 43和p3 8表达的影响。结果 脑室注射外缘性NGF后 ,GAP 43和p3 8表达较对照组有所增高 ,但差异无显著性 ( P >0 .0 5 )。结论 提示GAP 43和 p3 8是中枢神经系统损伤和再生的分子标志物 ,外源性NGF对GAP 43和 p3 8的调节有待于进一步研究。
Objective To study the effects of exogenous nerve growth factor (NGF) on expressions of growth associated protein 43 (GAP 43) and synaptophysin p38 after middle cerebral artery occlusion/reperfusion in rats. Methods 54 adult female rats were divided randomly into NGF group, controlled group and sham operated group. The rats received left middle cerebral artery occlusion of 2 hours. The sections of the brains which were subjected 6 hours and 1,3,7,14,21days of reperfusion, and were processed by immunochemistry with antibodies against GAP 43 and p38. Results The expressions of GAP 43 and p38 were slightly increased after NGF treatment, but the differences were not remarkable(P> 0.05 ). Conclusion It is suggested that there might be neuroplasticity of regeneration and repairment in central nervous system after injury. The regulative effects of exogenous NGF on GAP 43 and p38 need further exploration.
出处
《中华物理医学与康复杂志》
CSCD
2000年第4期201-203,共3页
Chinese Journal of Physical Medicine and Rehabilitation
基金
青岛市科委基金资助项目!(98 1 7)
关键词
神经生长因子
脑缺血再灌注
生长相关蛋白
P38
Nerve growth factors
Cerebral ischemia
Growth associated protein
Synaptophysin
Rats
